摘要
以鳀鱼鱼油和辛酸(CA)为底物,研发脂肪酶Lipozyme TL IM两步酶催化法(醇解反应和酯化反应)制备MLM结构三酰甘油酯。结果表明:在醇解反应过程中,Lipozyme TL IM催化鱼油制备2-单酰甘油酯的最佳含水量和乙醇与底物的摩尔比分别为5%~9%和32∶1。在此条件下,2-单酰甘油酯质量分数最高可达35%左右。在酯化反应过程中,Lipozyme TL IM催化2-单酰甘油酯与辛酸制备MLM结构三酰甘油酯的最适条件为:在正己烷溶剂体系下,催化温度为40℃,2-单酰甘油酯与辛酸的摩尔比为1∶3,催化时间为24 h。在此条件下,MLM结构三酰甘油酯的最大转化率为91.84%,其中CA-LSFAs(长链饱和脂肪酸)-CA(22.27%)和CA-PUFAs-CA(44.67%)是MLM结构三酰甘油酯的主要类型。这些结果表明,Lipozyme TL IM是一种有潜力的生物催化剂,催化鱼油制备MLM结构三酰甘油酯可应用于食品、医药等领域。
In this study,two-step enzymatic process(ethanolysis and esterification)mediated with Lipozyme TL IM as the biocatalyst was established to produce medium-long-medium structured triacylglycerols(MLM-STAGs)using anchovy oil and caprylic acid(CA).Results indicated that:In the ethanolysis reaction by Lipozyme TL IM,the highest 2-monoacylglycerols(2-MAGs)yield(around 35%)was obtained in 5%~9%of water content and 32∶1 of ethanol to fish oil molar ratio.In the esterification by Lipozyme TL IM,the greatest MLM-STAGs yield(91.84%)was achieved in 2-MAGs to CA molar ratio of 1∶3 at 40℃for 24 hours.The final MLM-STAGs mainly contained CA-LSFAs(long chain saturated fatty acid)-CA(22.27%)and CA-PUFAs-CA(44.67%).In all,Lipozyme TL IM was a potential biocatalyst for high-value MLM-STAGs products from fish oil for food,medicine and other fields.
作者
钟晨
何勇锦
Zheng Guo
陈必链
Zhong Chen;He Yongjin;Zheng Guo;Chen Bilian(College of Life Sciences,Fujian Normal University,Fuzhou 350117;Engineering Research Center of Ministry of Education of Industrial Microbiology,Fujian Normal University,Fuzhou 350117;Department of Engineering,University of Aarhus,Aarhus8000 Denmark)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2020年第10期141-147,154,共8页
Journal of the Chinese Cereals and Oils Association
基金
“十三五”海洋经济创新发展示范项目子课题(FZHJ04)。