摘要
采用自组装方法构建金磁微粒(Fe3O4@Au)复合纳米粒子,并表征其理化性能。评估Fe3O4@Au模拟酶的催化动力学过程,并基于其过氧化物模拟酶活性,优化出具有高灵敏度和选择性的检测过氧化氢体系。研究结果表明,Fe3O4@Au相比于天然过氧化物酶具有更小的Km数值;H2O2最佳检测条件为Fe3O4@Au浓度0.075 mg/mL、催化时间60 min和催化温度70℃;在最适检测体系下,H2O2浓度在0.005~0.08 mol/L时,吸光度数值与其具有良好线性关系,工作曲线方程为y=22.966x+0.689,检出限为4.9μmol/L。
Fe3O4@Au nanomaterials are prepared by self-assembly method,and their physical and chemical properties are characterized.The catalytic kinetic process of Fe3O4@Au mimetic enzyme is evaluated.Based on its peroxidase mimetic enzyme activity,a highly sensitive and selective detection system for hydrogen peroxide is optimized.The results show that the prepared Fe3O4@Au has smaller Km compared with natural peroxidase.The optimum conditions for detection of hydrogen peroxide are as follows:Fe3O4@Au concentration is 0.075 mg·m L-1,catalytic time is 60 min and catalytic temperature is kept at 70℃.Under the optimal detection system,the working curve equation of hydrogen peroxide is y=22.966x+0.689 when H2O2 concentration is in the range of 0.005-0.08 mol·L-1,and the detection limit is 4.9μmol·L-1.
作者
关桦楠
彭勃
龚德状
薛悦
张娜
刘晓飞
GUAN Hua-nan;PENG Bo;GONG De-zhuang;XUE Yue;ZHANG Na;LIU Xiao-fei(College of Food Engineering,Harbin University of Commerce,Harbin 150076,China)
出处
《现代化工》
CAS
CSCD
北大核心
2020年第7期230-234,共5页
Modern Chemical Industry
基金
国家自然科学基金项目(31201376、31370649)
黑龙江省自然科学基金项目(C2016034)
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016060)
中国博士后基金资助项目(2014T70304、2013M531009)
黑龙江省博士后资助项目(LBH-Z13002)
哈尔滨商业大学科研项目(17XN026)。
