摘要
目的:探讨MTHFR和MTRR基因多态性与多囊卵巢综合征(PCOS)的相关性,并为补充叶酸治疗PCOS提供理论支持。方法:通过病例对照研究,荧光定量多聚合酶链反应检测375例受试者(PCOS 150例,对照组225例)的MTHFR C677T、A1298C和MTRR A66G基因多态性,化学免疫发光法测定叶酸水平,循环酶法测定同型半胱氨酸水平。结果:MTHFR A1298C基因多态性中,PCOS组有较高比例的突变基因型CC,差异有统计学意义(χ^2=23.481,P<0.05,OR=5.043,95%CI为2.261~11.249)。MTRR A66G基因多态性中,PCOS组的突变基因型GG比例较高,差异有统计学意义(χ^2=8.029,P<0.05,OR=2.146,95%CI为1.051~4.382)。两组的叶酸、同型半胱氨酸水平比较,差异有统计学意义(P<0.05)。结论:MTHFR A1298C、MTRR A66G基因多态性可能是PCOS的危险因素,叶酸和同型半胱氨酸水平对PCOS有影响。
Objective:To investigate the correlation between MTHFR and MTRR gene polymorphism and polycystic ovary syndrome(PCOS),and to provide theoretical support for folic acid supplementation in the treatment of PCOS.Methods:375 subjects were examined by fluorescence quantitative multiple polymerase chain reaction(PCOS 150 cases,the control group 225cases),MTHFR C677T,A1298C and MTRR A66G gene polymorphisms were detected.Results:In MTHFR A1298C gene polymorphism,the PCOS group had a high proportion of mutated genotype CC(χ^2=23.481,P<0.05,OR=5.043,95%CI为2.261~11.249).In the MTRR A66G gene polymorphism,the GG ratio of mutated genotypes in the PCOS group was higher,and the difference was statistically significant(χ^2=8.029,P<0.05,OR=2.146,95%CI 1.051~4.382).In addition,there were statistically significant differences in folic acid and homocysteine levels between the two groups(P<0.05).Conclusion:MTHFR A1298C and MTRR A66G polymorphisms may be risk factors for PCOS,and folic acid and homocysteine levels may influence PCOS.
作者
冯婉琴
潘沅
张懿
刘敏娟
邓月秀
马颖
Feng Wanqin;Pan Yuan;Zhang Yi(Department of Obstetrics and Gynaecology,Zhujiang Hospital of Southern Medical University,Guangzhou 510282;Department of Clinical Laboratory,Zhujiang Hospital of Southern Medical University,Guangzhou 510282)
出处
《现代妇产科进展》
CSCD
北大核心
2020年第11期825-829,共5页
Progress in Obstetrics and Gynecology
基金
国家自然科学基金项目(No:81701418)。
