摘要
目的研究补肾强身片对多囊卵巢综合征(PCOS)模型大鼠的干预作用及机制。方法将50只大鼠灌胃来曲唑混悬液(1 mg/kg,每天1次,连续21 d)复制PCOS模型。将造模成功的大鼠分为模型组、阳性对照组(炔雌醇环丙孕酮片0.2 mg/kg+盐酸二甲双胍片230 mg/kg)和补肾强身片低、中、高剂量组(189、378、756 mg/kg),每组10只。另取10只未造模大鼠作为正常组。各组大鼠给予相应药物干预,每天1次,连续30 d。末次给药24 h后,检测大鼠血清中雌二醇(E_(2))、睾酮(T)、促性腺激素释放激素(GnRH)、促卵泡激素(FSH)和促黄体生成素(LH)水平,计算大鼠卵巢系数,观察大鼠卵巢组织病理形态学变化,检测大鼠卵巢组织中磷脂酰肌醇3激酶(PI3K)、磷酸化蛋白激酶B(p-Akt)、磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)和葡萄糖转运蛋白4(GLUT4)的表达水平以及PI3K、Akt、mTOR、GLUT4 mRNA表达水平。结果与正常组比较,模型组大鼠卵巢系数、卵巢囊性病变评分均显著增加(P<0.05),血清中T、GnRH、LH水平均显著升高(P<0.05),血清中FSH、E_(2)水平和卵巢组织中PI3K、p-Akt、pmTOR、GLUT4蛋白表达水平及PI3K、Akt、mTOR、GLUT4 mRNA表达水平均显著降低(P<0.05),卵巢组织中闭锁卵泡及无卵丘的囊性卵泡数量增多,卵泡颗粒细胞层数减少。与模型组比较,补肾强身片中、高剂量组大鼠上述指标水平均显著逆转(P<0.05),低剂量组上述大部分指标水平显著逆转(P<0.05),卵巢组织病变均不同程度改善。结论补肾强身片可调节PCOS模型大鼠性激素分泌水平,改善卵巢囊性病变,其作用机制可能与上调PI3K/Akt/mTOR、PI3K/Akt/GLUT4两条信号通路有关。
OBJECTIVE To study the intervention effect of Bushen qiangshen tablet on polycystic ovary syndrome(PCOS)model rats and its mechanism.METHODS Totally 50 rats were given letrozole suspension(1 mg/kg,once a day,for consecutive 21 d)instragastrically to induce PCOS model.Model rars were randomly divided into model group,positive control group(Ethinylestradiol and cyproterone acetate tables 0.2 mg/kg+Metformin hydrochloride tables 230 mg/kg),Bushen qiangshen tablet low-dose,medium-dose and high-dose groups(189,378,756 mg/kg),with 10 rats in each group.Another 10 healthy rats were included in normal group.Each group was given the corresponding drugs,once a day,for consecutive 30 d.Twenty-four hours after the last administration,serum levels of estrogen(E_(2)),testosterone(T),gonadotropin-releasing hormone(GnRH),follicle-stimulating hormone(FSH)and luteinizing hormone(LH)were measured.The ovary index was calculated,and pathological changes of ovary were observed.The protein expressions of phosphoinositide 3-kinase(PI3K),phosphorylated protein kinase B(p-Akt),phosphorylated mammalian target of rapamycin(p-mTOR)and glucose transporter 4(GLUT4)in ovary were detected,and mRNA expressions of PI3K,Akt,mTOR and GLUT4 in ovary were detected.RESULTS Compared with normal group,ovarian index and ovarian cystic disease score were significantly increased in model group(P<0.05),and serum levels of T,GnRH and LH were significantly increased(P<0.05);serum levels of FSH and E_(2),protein expressions of PI3K,p-Akt,p-mTOR and GLUT4,and mRNA expression of PI3K,AKT,mTOR and GLUT4 in ovarian tissue were all significantly decreased in the model group(P<0.05);in ovarian tissue,the number of atresia follicles and non-cumulus cystic follicles increased,and the number of granulosa cell layers decreased.Compared with model group,above indexes of Bushen qiangshen tablet medium-dose and high-dose groups were reversed significantly(P<0.05),and most above indexes of low-dose group were reversed significantly(P<0.05),the pathological changes of ovarian
作者
张明昊
高一盈
董文霞
薛鹏坤
马伟洋
张大伟
马丽亚
ZHANG Minghao;GAO Yiying;DONG Wenxia;XUE Pengkun;MA Weiyang;ZHANG Dawei;MA Liya(Medical College,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Dept.of Obstetrics and Gynecology,the Third Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou 450008,China;Clinical Skills Training Center,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China)
出处
《中国药房》
CAS
北大核心
2022年第21期2632-2637,2653,共7页
China Pharmacy
基金
河南省科技发展计划项目(No.222102310139)
河南中医药大学科研苗圃工程项目(No.MP2020-98)。
