摘要
背景与目的最近研究发现DNA修复基因多态性可以影响到肿瘤的易感性,因此通过不同的DNA修复基因可筛选出肿瘤的易感人群,从而有望达到肿瘤的早期预防、诊断和治疗。本研究旨在分析DNA修复基因ERCC1多态性及其与肺癌易感性的关系。方法采用病例-对照研究,收集上海肺科医院原发性肺癌患者291例为病例组,同期住院的非肿瘤患者273例作为对照组,并进行流行病学调查。应用Taqman探针结合实时荧光PCR方法分析病例组和对照组的ERCC1基因T118C的多态性分布,比较不同基因型与肺癌易感性的关系,以及基因多态性与吸烟对肺癌的交互作用。结果在不吸烟人群中,ERCC1基因118位点3种基因型在病例组和对照组人群中分布差异有统计学意义(χ2=11.19,P<0.01)。在不吸烟人群中,与携带野生纯合基因型(C/C)相比,携带突变纯合基因型(T/T)者患肺癌的风险会增加,其校正OR值为3.16(95%CI:1.29-7.73,P<0.01)。以携带野生纯合基因型(C/C)且不吸烟者作为参照,吸烟>25包-年且携带野生纯合基因型(C/C)或杂合基因型(C/T)者患肺癌的风险均会提高,其校正OR值分别为2.62(95%CI:1.54-4.44)、2.41(95%CI:1.36-4.26,P<0.01)。以携带野生基因型者作为参照,携带突变纯合基因型者患腺癌的风险度会增加,其OR值为2.29(95%CI:1.05-4.78,P=0.03)。结论DNA修复基因ERCC1118C/T多态性可能对肺癌易感性产生影响,并可能与吸烟有一定的协同作用。
Background and objective The recent studies have demonstrated that polymorphisms of DNA repair genes can impact the tumor susceptibility. So the susceptible group can be chosen through analysis of different genotype of DNA repair genes and maybe get the early prevention, early diagnosis and early treatment. The aim is to analyze the polymorphisms of DNA repair gene ERCC1 and relationship between genetic variations and susceptibility to lung cancer. Methods A case-control group study of 291 patients with lung cancer and 273 cancer-free subjects as a control group was conducted to detect ERCC1 polymorphisms at loci. Genotypes of ERCC1 were analyzed by real time PCR techniques using Taqman probes. Results In never smoking people, a significant difference was found in the 3 genotype （C/C,C/T,T/ T） between cases and controls （X^2=11.19, P〈0.01）. In never smoking people, compared with homozygous genotype （C/C）, ERCC1 codon 118 homozygous genotype （T/T） might increase the risk of lung cancer, the adjusted OR is 3,16 （95%CI： 1.29-7.73, P〈0.01）.Compared with homozygous genotype （C/C） and never smoking ,those with smoking dose〉25 pack-year and homozygous genotype （C/C） or heterozygous genotype （C/T） might increase the risk of lung cancer, the adjusted OR are 2.62 （95%CI： 1.54-4.44）, 2.41 （95%CI： 1.36-4.26, P〈0.01） respectively. Compared with homozygous genotype （C/C）, homozygous genotype （T/T） might increase the risk adenocarcinoma （OR=2.29, 95%CI： 1.05-4.78, P=0.03）. Conclusion The results demonstrate that genetic polymorphism of ERCC1 DNA repair gene might contribute the susceptibility to lung cancer and have synergistic effect with smoking.
出处
《中国肺癌杂志》
CAS
2008年第2期183-188,共6页
Chinese Journal of Lung Cancer
基金
上海市科委重大项目基金(No.06DZ19502)资助~~
关键词
肺肿瘤
基因多态性
疾病遗传易感性
Lung neoplasms Genetic polymorphism Genetic predisposition to disease
