摘要
目的:探讨RNA干扰沉默Survivin表达对131I抑制甲状腺癌FTC-133细胞生长的作用及其机制。方法:采用脂质体法将Survivin-siRNA及其阴性对照NC-siRNA转染至干扰组和NC组细胞中,Real-time PCR和Western blot检测Survivin mRNA和蛋白的表达;以131I孵育后,MTT法、流式细胞仪检测Survivin对FTC-133细胞增殖、周期和凋亡的影响,Western blot检测细胞中CDK2、Cyclin E、Bax和Bcl-2蛋白的表达变化。结果:转染Survivin-siRNA成功沉默FTC-133细胞中Survivin mRNA和蛋白的表达,抑制FTC-133细胞增殖,诱导细胞阻滞于G0/G1期和细胞凋亡,并下调细胞中CDK2、Cyclin E和Bcl-2蛋白的表达,上调Bax蛋白的表达;131I孵育后,干扰组细胞中的上述变化较NC组明显增强。结论:沉默Survivin表达可提高131 I对FTC-133细胞增殖的抑制作用和对细胞凋亡的促进作用,其作用机制可能与下调CDK2、Cyclin E、Bcl-2和上调Bax蛋白的表达有关。
Objective:To investigate the effect of RNA interference silencing Survivin expression on 131I inhibiting the growth of thyroid cancer FTC-133 cells and its mechanism.Methods:Survivin-siRNA was transfected into FTC-133 cells by liposome method.The expression of Survivin mRNA and protein were detected by Real-time PCR and Western blot.After incubation with 131I,the effects of silenced Survivin on proliferation,cycle and apoptosis of FTC-133 cells were measured by MTT and flow cytometry,and the expression of CDK2,Cyclin E,Bax and Bcl-2 proteins in FTC-133 cells were tested by Western blot.Results:The expression of Survivin mRNA and protein in FTC-133 cells were successfully silenced,and the proliferation of FTC-133 cells was inhibited.Cell was arrested at G0/G1 phase and cell apoptosis were induced,and the expression of CDK2,Cyclin E and Bcl-2 proteins in cells were down-regulated,and the expression of Bax protein was up-regulated after Survivin-siRNA transfection successfully.After 131I incubation,the above changes in interference group were significantly enhanced compared with NC group.Conclusion:Silencing Survivin expression can enhance the inhibitory effect of 131I on proliferation and promotion of apoptosis of FTC-133 cells.Its mechanism may be related to down-regulation of CDK2,Cyclin E,Bcl-2 and up-regulation of Bax protein expression.
作者
满聪
MAN Cong(Department of Nuclear Medicine,Zaozhuang Mining Group Central Hospital,Shandong Zaozhuang 277800,China)
出处
《现代肿瘤医学》
CAS
2020年第21期3684-3688,共5页
Journal of Modern Oncology
