摘要
珠子参作为一种珍稀药用植物,可以为我们提供珍贵的药物资源,而作为高等植物中最大转录因子之一的WRKY转录因子在珠子参中的研究鲜有报道。主要分析珠子参WRKY转录因子序列,首先从珠子参转录组数据中筛选出长度大于500 bp的WRKY基因,然后进行保守结构域分析,同时用这些序列与珠子参所含成分相似的人参属植物人参、三七、西洋参的WRKY转录因子进行多序列比对,可以预测序列之间的结构和功能,然后做系统发育分析,分析序列之间的同源关系,最后将具有WRKY保守结构域的序列与珠子参茎节与节间总差异表达中筛选出交集,将交集基因及其差异表达数据绘制出热图,可以得到各差异表达基因的关系及基因表达水平。结果显示,72条珠子参WRKY转录因子序列中共有45条序列具有WRKY保守结构域,其中包含8个I组WRKY转录因子、2个III组WRKY转录因子、32个II组WRKY转录因子,剩余3个锌指结构缺失的WRKY转录因子。从进化关系来看,其中有6个珠子参WRKY转录因子跟人参、西洋参、三七的亲缘关系比较近。从差异表达基因来看,大多数WRKY基因在珠子参的根茎的茎节中的表达水平较高,而在根茎的节间中WRKY基因表达水平较低。研究结果将为理解WRKY转录因子在珠子参根茎发育和皂苷合成过程中的作用提供理论依据。
As a rare medicinal plant,the Panax japonicus can provide us with precious medicinal resources.However,as one of the largest transcription factors in higher plants,WRKY transcription factor has not been reported so far.In this study,WRKY transcription factor sequences of Panax japonical were analyzed.Firstly,WRKY genes with a length of more than 500bp were screened out from the transcriptome number of Panax japlcus var,and then,conservative domain analysis was carried out.At the same time,the WRKY transcription factors of similar Panax ginseng,Panax notoginseng and Panax quinquefolium were compared by bioinformatics software,which can predict the structure and function of sequences,then do phylogenetic analysis,analyze the homologous relationship between sequences,and finally screen the intersection between sequences with WRKY conserved domain and the expression of total differences between stem nodes and internodes of Panax japlcus var.By drawing the heatmap of genes and their differential expression data,we can get the relationship and gene expression level of each differential expression gene.The results showed that there were 45 WRKY conserved domains in 72 WRKY transcription factors,including 8 WRKY transcription factors in group I,2 WRKY transcription factors in group III,32 WRKY transcription factors in group II,and 3 WRKY transcription factors with missing zinc finger structure.From the evolutionary point of view,there are 6 WRKY transcription factors are closely related to Panax ginseng,Panax notogenseng and Panax quinquefolium.From the perspective of differential expression genes,most of the WRKY genes were expressed at a higher level in the rhizome of Panax japonicus,but at a lower level in the stem nodes of Panax japonicus.The results of this study will provide an important theoretical basis for understanding the role of WRKY transcription factor in the development of rhizome and the synthesis of saponins.
作者
张瑞
蒋鹏
张祚
左天
张绍鹏
ZHANG Rui;JIANG Peng;ZHANG Zuo;ZUO Tian;ZHANG Shao-peng(School of Biology and Pharmaceutical Engineering,Wuhan Polytechnic University,Wuhan 430023,China)
出处
《武汉轻工大学学报》
2020年第4期32-37,58,共7页
Journal of Wuhan Polytechnic University
基金
湖北省自然科学基金面上项目(2018CFB565)。
关键词
珠子参
转录组
WRKY
转录因子
Panax japonicus
transcriptome
WRKY
transcript factor
