摘要
为查清长牡蛎(Crassostrea gigas)壳橙品系在不同生长阶段各生长性状的遗传力、遗传相关和表型相关,本实验通过平衡巢式设计和人工授精的方法,建立了30个全同胞家系,利用个体动物模型计算了长牡蛎壳橙品系幼虫期和稚贝期的壳高、壳长、壳宽和湿重的遗传参数。研究发现:幼虫在1~21日龄时壳高和壳长的遗传力分别为0.19±0.08~0.36±0.11和0.17±0.08~0.28±0.10,均为中等遗传力;稚贝在90~270日龄时壳高和壳长的遗传力分别为0.13±0.07~0.40±0.12和0.11±0.06~0.37±0.12,在270日龄时壳宽和湿重的遗传力分别为0.17±0.08和0.22±0.09。在不同生长阶段,各性状之间的遗传相关和表型相关均为正相关,其中壳高和湿重与其它性状间的遗传相关相对更高(分别为0.35±0.30~0.99±0.08和0.72±0.17~0.93±0.06),表明对这两个性状的直接选育将对其它生长性状起到间接促进作用。本研究获得了长牡蛎壳橙品系不同生长阶段的遗传参数,这些参数将为制定适宜的选育技术路线提供基础资料。
The Pacific oyster(Crassostrea gigas)is the most widely cultured shellfish in the world,and its shell coloration attracts a constant attention of worldwide breeders.We obtained orange variants in our selective breeding practice,and genetically stable strains through family selection.Due to the limited number of effective population size,potential inbreeding depression has become a concern.Slower growth and worse adaptive capacity to environmental stresses were observed in breeding practice.There is a considerable focus on improving selective breeding procedures for growth traits in the orange-shell strain.We used specimens of the orange-shell strain of C.gigas after seven generations of mass selection as parents to established 30 full-sib families(20 families survived eventually)in a nested design.The shell height and shell length of larvae were measured on days 1,6,11,16 and 21 post-hatch(30 individuals per family)at larvae stage and on ages of 90,180 and 270 days at juvenile stage.Besides,the shell width and wet weight were measured on age of 270 days.REML based on the animal model was used to estimate genetic parameters of orange-shell C.gigas at the larval and juvenile stages.The estimation of heritability varied between 0.19±0.08 and 0.36±0.11 for shell height and between 0.17±0.08 and 0.28±0.10 for shell length at larvae stage.At juvenile stage,the heritability estimations for shell height and shell length ranged from 0.13±0.07 to 0.40±0.12 and from 0.11±0.06 to 0.37±0.12,respectively,and that for shell width and wet weight was 0.17±0.08 and 0.22±0.09,respectively.Although the heritability estimations in this study are slightly lower than those of previous studies,it is possible to improve the growth traits of orange shell strain by selection.All phenotypic and genetic correlations among shell height,shell length,shell width and wet weight were positive.Genetic correlations were higher between shell height,wet weight and the other growth traits,which ranged from 0.35±0.30 to 1.00±0.09 and from 0
作者
方佳峰
李琪
FANG Jia-Feng;LI Qi(The Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China;Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第11期38-44,共7页
Periodical of Ocean University of China
基金
泰山学者种业计划专家项目
山东省农业良种工程项目(2017LZGC009)资助。
关键词
长牡蛎
壳橙品系
幼虫期
稚贝期
生长性状
遗传参数
遗传力
遗传相关
表型相关
Pacific oyster
orange-shell strain
larvae stage
juvenile stage
growth trait
genetic parameter
heritability
genetic correlations
phenotypic correlations
