摘要
为建立山地野生黄精高效组培增殖和生根体系,给其工厂化、规模化育苗提供可行的关键技术方案,本文以山地野生黄精无菌种子为试验材料,研究不同培养基种类、不同激素浓度对黄精种子萌发、继代、增殖、生根、炼苗移栽的效果.结果表明:黄精种子萌发启动的最佳培养基为MS+6-BA(0.2 mg/L)+2,4-D(0.2 mg/L)+GA 3(2.0 mg/L),种子萌芽率为20%,90 d后芽约有0.50 mm长,生长情况良好;愈伤组织继代培养的最佳培养基为MS+6-BA(4.0 mg/L)+2,4-D(0.2 mg/L)+TDZ(0.2 mg/L),愈伤组织长势最好,芽点增殖多,苗壮;增殖培养的最佳培养基为MS+6-BA(2.0 mg/L)+TDZ(0.2 mg/L)+NAA(0.2 mg/L),芽生长状况良好,伸长较大,叶色绿,苗壮;生根诱导培养的最佳培养基为1/2 MS+TDZ(0.01 mg/L)+NAA(0.2 mg/L),生根率最高为70%,根数、根长最好,分别为14条、0.60 cm;炼苗移栽后4~8条根须移栽的成活率最高达92%.实验结果认为,以黄精无菌种子为培养对象可短时间获得大量黄精组培苗,有利于解决种植生产上的种苗短缺问题.
In order to establish a high-efficiency tissue culture propagation and rooting system for wild polygonatum sibiricum,and provide a feasible key technical solution for its factory and large-scale nursery,this article uses sterile wild polygonatum sibiricum seeds as test materials to study the effects of seed germination,subculture,multiplication,rooting and transplanting of refined seeds.The results showed that the best medium for seed germination and start of polygonatum sibiricum was MS+6-BA(0.2 mg/L)+2,4-D(0.2 mg/L)+GA 3(2.0 mg/L),the seed germination rate was 20%,and the buds were about 90 ds later.It is 0.50 mm long and grows well.The best medium for subculture of callus is MS+6-BA(4.0 mg/L)+2,4-D(0.2 mg/L)+TDZ(0.2 mg/L),callus the growth is the best,the bud spots are multiplying,and the seedlings are strong;the best medium for multiplying culture is MS+6-BA(2.0 mg/L)+TDZ(0.2 mg/L)+NAA(0.2 mg/L),the buds grow well and elongate large,green leaves,strong seedlings.The best medium for rooting induction culture is 1/2 MS+TDZ(0.01 mg/L)+NAA(0.2 mg/L),the rooting rate is up to 70%,and the root number and root length are the best,respectively,14 pieces,0.60 cm.The survival rate of transplanting 4-8 roots after transplanting seedlings is up to 92%.The experimental results believe that using sterile seeds of polygonatum sibiricum as a cultivation object can obtain a large number of tissue culture seedlings of polygonatum sibiricum in a short time,which is beneficial to solve the problem of shortage of seedlings in planting and production.
作者
田怀
侯娜
Tian Huai;Hou Na(Guizhou Forestry School,Guiyang 550201,China;Guizhou Academy of Forestry,Guiyang 550201,China)
出处
《南京师大学报（自然科学版）》
CAS
CSCD
北大核心
2020年第3期129-135,共7页
Journal of Nanjing Normal University(Natural Science Edition)
基金
贵州乡土珍稀花卉研发团队服务企业行动计划项目(黔科合平台人才[2016]5711)
贵州省科技平台项目“贵州省林木标本数据库及网络共享平台建设”(黔科平台[2012]4002).
关键词
黄精
组织培养
快速繁殖
种子
Polygonatum sibiricum
tissue culture
rapid propagation
seed
