摘要
结核病分子流行病学主要利用分子流行病学分型技术结合流行病学资料研究结核病的传播和流行特点。全基因组测序利用新一代生物信息技术获得物种个体或群体的基因图谱,具有高分辨率、高通量、结果精确和周期短等优势,能弥补传统基因分型技术的不足,已广泛应用于结核病分子流行病学研究。本文对全基因组测序的发展以及在鉴别菌株类型及谱系、鉴定传播方向、疫情追踪和菌株进化等方面的应用进行综述,为结核病的防控和研究提供参考。
Tuberculosis molecular epidemiology mainly applies strain genotyping technology combined with epidemiological data to explore the transmission characteristics.Whole genome sequencing obtains the genomes of species using new biological information technology,whose high resolution,high throughput,high precision and short cycle time can make up for the shortcomings of traditional gene typing technology,and has been widely used in the tuberculosis molecular epidemiology.In this paper,we reviewed the development of whole genome sequencing recently and its application in the identification of strain types and pedigrees,identification of transmission direction,epidemic tracing and strain evolution,so as to provide reference for the control of tuberculosis and related research.
作者
郭海萍
尚媛媛(综述)
李姗姗
逄宇(审校)
GUO Haiping;SHANG Yuanyuan;LI Shanshan;PANG Yu(National Clinical Laboratory on Tuberculosis,Beijing Chest Hospital affiliated to Capital Medical University,Beijing 101149,China)
出处
《预防医学》
2020年第9期899-903,共5页
CHINA PREVENTIVE MEDICINE JOURNAL
基金
北京市医院管理中心“登峰”人才培养计划(DFL20191601)。
关键词
结核病
结核分枝杆菌
全基因组测序
分子流行病学
tuberculosis
Mycobacterium tuberculosis
whole genome sequencing
molecular epidemiology
