摘要
为探明干旱区栽培黑果枸杞叶片光合-CO2响应特征及果实药效成分对施氮量的响应,该试验设置5个不同施氮(尿素)水平处理(0、50、100、150和200 g·株^-1),测定各处理植株在不同CO2浓度下叶片净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、水分利用率(WUE)、胞间CO2浓度(Ci)和果实中总多糖、总黄酮、花色苷、原花青素的含量,并通过直角双曲线修正模型拟合得到羧化速率(η)、最大净光合速率(Pnmax)、光呼吸速率(Rp)、CO2补偿点(CCP)和CO2饱和点(CSP)等参数。结果表明:(1)适量施氮对黑果枸杞叶片η、Pnmax、Rp、CCP和CSP有明显的影响。当施氮量为100 g·株^-1时,叶片η和Rp最大、CCP最小;施氮量为100~150 g·株^-1时,叶片CSP最大;施氮量为150~200 g·株^-1时,叶片Pnmax最大。(2)适量施氮对黑果枸杞果实中主要药效成分含量有明显的影响。施氮量为200 g·株^-1时,果实总多糖含量最大;施氮量为150~200 g·株^-1时,总黄酮含量最大;施氮量为50~150 g·株^-1时,花色苷含量最大;施氮量为100~200 g·株^-1时原花青素含量最大。综合分析发现,干旱区栽培黑果枸杞叶片η、Rp、CCP、CSP及果实多糖和总黄酮含量以每年施氮100~150 g·株^-1时最佳,其叶片Pnmax和果实花色苷和原花青素含量以每年施氮150~200 g·株^-1时最佳。
We explored the response of photosynthetic-CO2 response curve and fruit medicinal components of Lycium ruthenicum cultivated in arid areas to nitrogen(urea,N,46%)application.The experiment had five different N application rates[0(CK),50,N2:100,150 and 200 g·strain^-1].The net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),water use efficiency(WUE),intercellular CO2 concentration(Ci)and the contents of total polysaccharides,total flavonoids,anthocyanins,proanthocyanidins in the fruit were measured,and the parameters including carboxylation rate(η),maximum net photosynthesis rate(Pnmax),photorespiration rate(Rp),CO2 compensation point(CCP)and CO2 saturation point(CSP)were obtained by fitting a right-angle hyperbolic correction model.The results showed as follows:(1)appropriate N application rate had certain effects onη,Pnmax,Rp,CCP and CSP in leaves of L.ruthenicum.When N application rate was 100 g·strain^-1,ηand Rp were the largest,and CCP was the smallest.When N application rate was 100~150 g·strain^-1,CSP was the largest.When N application rate was 150-200 g·strain^-1,Pnmax was the largest.(2)Appropriate N application rate had certain effects on the contents of the main medicinal components in fruits of L.ruthenicum.When N application rate was 200 g·strain^-1,the total polysaccharide content was the largest.The total flavonoid content was the largest when N application rate was 150-200 g·strain^-1.The anthocyanin content was the largest when N application rate was 50-150 g·strain^-1.The proanthocyanidin content was the largest when N application rate was 100-200 g·strain^-1.For comprehensive consideration,the best L.ruthenicum planting conditions in arid areas are the optimum ofη,Rp,CCP,CSP,contents of polysaccharides and total flavonoids when N application rate at 100-150 g·plant^-1,and the optimum of Pnmax,contents of anthocyanins and procyanidins when N application rate at 150-200 g·plant^-1 per year.
作者
马兴东
郭晔红
李梅英
马旭东
张祝莉
朱文娟
Deborah
MA Xingdong;GUO Yehong;LI Meiying;MA Xudong;ZHANG Zhuli;ZHU Wenjuan;Deborah(College of Agronomy, Gansu Agricultural University/Institute of Traditional Chinese Medicine/Gansu Provincial Key Laboratory of Arid Land Crop Science, Lanzhou 730070, China;2Gansu Endangered Animal Protection Center, State Forestry Administration, Wuwei, Gansu 733000, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2020年第7期1209-1218,共10页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31860349,31860102)
甘肃省科技计划(18YF1NA072)
甘肃林业科技推广项目。
关键词
干旱区
黑果枸杞
施氮量
光合-CO2响应曲线
药效成分
arid area
Lycium ruthenicum Murr.
N application rate
photosynthesis-CO2 response curve
medicinal components
