摘要
为探究多次净风和风沙流胁迫下黑果枸杞(Lycium ruthencium)适应能力及其响应机制,以两年生黑果枸杞实生苗为材料,采用风洞模拟试验,在9 m·s^(-1)的风速下,于第1、3、5天08:00进行20 min的净风和风沙流吹袭胁迫,观察记录叶片形态变化,并于第1、3、5天胁迫结束30 min后及第7天同一时间点采集黑果枸杞叶片,测定分析膜透性和膜保护系统相关指标。结果表明,净风和风沙流胁迫导致黑果枸杞部分叶片出现褐色斑块、萎蔫,风沙流伤害大于净风;净风胁迫下,叶片丙二醛(MDA)、可溶性糖(SS)先增后减,过氧化物酶(POD)先减后增,可溶性蛋白(SP)波动减小,膜透性、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、脯氨酸(Pro)呈增加趋势;风沙流胁迫下SOD先减后增,SS、SP先减后增再减,MDA、POD、膜透性、CAT、Pro的变化趋势均与净风胁迫一致。研究结果对于揭示黑果枸杞对风和风沙危害的逆境适应机制具有重要意义。
To explore the adaptive capacity and response mechanism of Lycium ruthenicum under multiple net-wind and wind-sand flow stress,two-year-old potted seedlings of L.ruthenicum were used.Using the wind tunnel simulation test,the seedlings were stressed by net-wind and wind-sand flow at 8:00 am for 20 mins on the 1 st,3 rd,and 5 th day at 9 m·s^(-1)wind speed.Under stress,the morphological changes of the leaves were observed and recorded.The leaves were collected 30 mins after application of the stress on the 1 st,3 rd,and 5 t h days and during the same time on the 7 th day to determine and analyze the relevant indices of membrane permeability and membrane protection system.The results showed that some leaves of L.ruthenicum exhibited brown patches and wilting under the net-wind and wind-sand flow stress,and the damage caused by wind-sand flow was significantly higher than that by net-wind.Under net-wind stress,malondialdehyde(MDA)and soluble sugar(SS)increased initially and then decreased;peroxidase(POD)decreased initially and then increased,soluble protein(SP)fluctuation decreased;and membrane permeability,superoxide dismutase(SOD),and catalase(CAT)and proline(Pro)increased.Under wind-sand flow stress,SOD decreased initially and then increased;SS and SP decreased initially and then increased and then decreased;and as regards the variation trends of MDA,POD,membrane permeability,CAT and Pro levels were identical to those under net-wind stress.The results of this study are of great significance for revealing the adversity adaptation mechanism of L.ruthenicum to wind and wind-sand damage.
作者
党绪
马瑞
魏林源
张莹花
侍新萍
马彦军
DANG Xu;MA Rui;WEI Linyuan;ZHANG Yinghua;SHI Xinping;MA Yanjun(Forestry College,Gansu Agricultural University,Lanzhou 730070,Gansu,China;State Key Laboratory Breeding Base of Desertification and Aeolian Sand Disaster Combating,Gansu Desert Control Research Institute,Lanzhou 730070,Gansu,China)
出处
《草业科学》
CAS
CSCD
北大核心
2022年第4期740-746,共7页
Pratacultural Science
基金
不同地理种源野生黑果枸杞对风沙流胁迫的耐性及适应机制(31760244)。
关键词
黑果枸杞
净风
风沙流
膜透性
保护酶
渗透调节物质
生理响应
Lycium ruthenicum
net-wind
wind-sand flow
membrane permeability
protective enzyme
osmotic regulation substances
physiological response
