摘要
【目的】探讨不同温度胁迫条件下3个烤烟品种抗氧化特性变化特征及其对烟叶淀粉降解的影响。【方法】采用电热式温湿度自控烤烟箱,以红花大金元、K326和云烟87中部叶为材料,在不同温度胁迫(低温中湿T36,中温中湿T38c和T38r,高温中湿T40)条件下观测烘烤过程中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性变化规律和淀粉降解动态。【结果】3个品种淀粉降解速率大小依次为T38c处理>T38r处理>T40处理>T36处理。淀粉降解速率与抗氧化酶活性密切相关,淀粉降解速率随着SOD和POD活性的增加而增加。3个品种的POD和SOD平均活性表现为:T40处理>T38c处理>T38r处理>T36处理。【结论】中高温胁迫能促进淀粉降解,在烟叶烘烤中可适当提高变黄期烘烤温度。
[Purpose]To investigate the effect of temperature stress on the starch degradation of tobacco,and provide a theoretical basis for the production of high quality tobacco leaves.[Method]The middle leaves of Honghuadajinyuan(HD),K326 and Yunyan87 were used as the materials to investigate the antioxidant enzyme activity(SOD,POD and CAT)and starch degradation under different temperature stress treatments(T36,T38c,T38r and T40)by using the electric-heated flue-curing barn under the temperature and humidity self-controlled.[Results]The starch degradation rate in the three varieties were:T38r treatment>T38c treatment>T40 treatment>T36 treatment.The rate of starch degradation was correlated with antioxidant enzyme activity.Correlation analysis showed that the starch degradation rate increased with SOD and POD activities increase.In the three varieties,POD enzyme activity and SOD enzyme activity were:T40 treatment>T38c treatment>T38r treatment>T36 treatment.[Conclusion]In curing of flue-cured tobacco,considering increased the temperature in the yellowing stage can be feasible to provide a theoretical basis for the production of high quality tobacco leaves.
作者
欧阳铖人
杨焕文
王戈
王娜
OUYANG Chengren;YANG Huangwen;WANG Ge;WANG Na(Collage of Tobacco Science,Yunnan Agricultural University,Kunming 650201,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2020年第5期804-809,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
中国烟草总公司云南省烟草公司科技计划重点专项项目(2019530000241012)。
关键词
烟叶
烘烤
温度胁迫
淀粉
抗氧化酶
flue-cured tobacco
curing
temperature stress
starch
antioxidant enzyme activity
