摘要
采用纤维素高效降解真菌Peniophoraintranata和Sarocladiumstrictum分别制备单一菌剂(菌剂A、B)以及二者混合菌剂(菌剂C),分3种剂量对胡桃楸、兴安落叶松及胡桃楸×兴安落叶松混交3种不同地表可燃物基质进行喷洒,每月取样,测定基质失质量以及综纤维素降解动态,并分析施加不同菌剂和不同剂量对可燃物基质综纤维素降解率的影响。结果表明:①施加菌剂的3种基质综纤维素降解效果均优于对照组,对于施加同种剂型的可燃物,不同剂量使可燃物基质综纤维素降解率由高到低排序为高浓度、中浓度、低浓度、对照;②3种可燃物基质综纤维素呈现出相同的降解规律,降解率由高到低表现为菌剂C、菌剂B、菌剂A、对照,即混合菌剂降解效果优于单一菌剂;③胡桃楸、兴安落叶松、混交基质综纤维素降解率与其减少质量均呈正相关,且相关关系达到显著水平(α=0.01),一元线性回归拟合优度分别为86.96%、95.75%、90.97%。综合以上结果可以得出,混合菌剂降解可燃物综纤维素的能力更强,且可燃物综纤维素降解率与可燃物载量密切相关,野外喷洒该菌剂能够有效降低森林地表可燃物载量,从而达到降低森林火险、预防森林火灾发生的目的。
The efficient cellulose-degrading fungi Peniophora intranata and Sarocladium strictumwere used to prepare the single microbial agents(microbial agent A,B)and a mixed microbial agent(microbial agent C).Three different surface fuel substrates of Juglans mandshurica,Larix gmelinii and theirmix were sprayed in three dosages.Samples were taken every month to determine the substrate weight loss and the holo-cellulose degradation dynamics,and to analyze the effect of applying different microbial agents and different doses on the fuelsubstratesholo-cellulose degradation rate.It was found that firstly,the degradation rate of holo-cellulose in the three substrates with different dosages was higher than that of the control,which showed by regularities in descending order of high concentration,medium concentration,low concentrationand control.Secondly,holo-cellulose of the three fuel substrates showed the same degradation law,and the degradation rate wasin descending order ofmicrobial agent C,microbial agent B,microbial agent A andcontrol,that is,the mixed microbial agent had better degradation effect than the single microbial agents.Thirdly,there was a positive correlation between the holo-cellulose degradation rate and reduced mass of Juglans mandshurica,Larix gmelinii and theirmixed substrates,and the correlation reached a significant level(α=0.01).The goodness of fit of univariate linear regression was 86.96%,95.75%,and 90.97%,respectively.Based on the above results,it can be concluded that the mixed microbial agent has a stronger ability to degrade substrateholo-cellulose,and the degradation rate of substrate holo-cellulose is closely related to the fuel load.Spraying the microbialagent in the field can effectively reduce the surface fuel loads,whichcan prevent and reduce forest fires.
作者
孙思琦
樊晓亮
宁吉彬
邸雪颖
杨光
Sun Siqi;Fan Xiaoliang;Ning Jibin;Di Xueying;Yang Guang(Key Laboratory of Sustainable Forest Ecosystem Management,Ministry of Education,Northeast Forestry University,Harbin 150040,P.R.China;Hebei Wuling Mountain National Nature Reserve Management Center)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2020年第8期61-65,共5页
Journal of Northeast Forestry University
基金
“十三五”国家重点研发计划子课题(2017YFD0600106-2)
国家自然科学基金面上项目(31870644)
中央高校基本科研业务费专项资金项目(2572019CP10)。
关键词
纤维素
真菌
野外降解
森林地表可燃物
Cellulose
Fungi
Degradation in field
Forest surface fuel
