摘要
目的探讨ANA、LKM-1、AMA M2、LC-1及SLA/LP联合检测在乙肝病毒感染分型中的应用研究。方法选取2015年1月~2019年5月我院传染科35例乙肝合并自身免疫性肝病患者作为观察组,同时选取65例乙肝病毒感染患者作为对照组。观察两组血清抗核抗体(ANA)、抗肝肾微粒体Ⅰ型抗体(LKM-1)、抗线粒体抗体(AMA M2)、抗肝胞浆Ⅰ型抗体(LC-1)、抗可溶性肝抗原/肝胰抗原抗体(SLA/LP)阳性率,对结果进行分析。结果观察组中ANA的阳性率(35.00%)高于对照组(21.86%),观察组中LKM-1、AMA M2、LC-1及SLA/LP的阳性率均显著高于对照组(P<0.05),差异均有统计学意义(P<0.05)。对照组ANA阳性主要为核颗粒型,主要在1:100的血清稀释度中检出,观察组ANA阳性主要为核均质性,主要在1:1000的血清稀释度中检出,两种核型稀释度组间差异有统计学意义(P<0.05)。结论多项自身抗体联合检测对乙肝及自身免疫性肝病的抗体检测全面,ANA检测是初筛试验,LKM-1、AMA M2、LC-1及SLA/LP等是确证试验,有助于临床诊断及指导用药。
Objective To investigate the application of combined detection of ANA,LKM-1,AMA M2,LC-1 and SLA/LP in typing of hepatitis B virus infection.Methods From January 2015 to May 2019,35 patients with hepatitis B and autoimmune liver disease in the Department of Infectious Diseases of our hospital were selected as the observation group,and 65 patients with hepatitis B virus infection were selected as the control group.The positive rates of serum anti-nuclear antibody(ANA),anti-liver and kidney microsome type I antibody(LKM-1),anti-mitochondrial antibody(AMA M2),anti-hepatic cytoplasmic type I antibody(LC-1),and anti-soluble liver antigen/liver-pancreas(SLA/LP)autoantibody of two groups were observed.The results were analyzed.Results The positive rate of ANA in the observation group(35.00%)was higher than that in the control group(21.86%).The positive rates of LKM-1,AMA M2,LC-1 and SLA/LP in the observation group were significantly higher than those in the control group(P<0.05),and the differences were statistically significant(P<0.05).The ANA positive in the control group was mainly nuclear particle type,mainly detected at 1:100 serum dilution,and the ANA positive in the observation group was mainly nuclear homogeneity,mainly detected at 1:1000 serum dilution.The difference in two karyotype dilutions between the groups was statistically significant(P<0.05).Conclusion The combined detection of multiple autoantibodies for the detection of hepatitis B and autoimmune liver diseases is comprehensive.The ANA test is a preliminary screening test.LKM-1,AMA M2,LC-1 and SLA/LP are confirmatory tests,which are helpful for clinical diagnosis.And guide medication.
作者
谢璟
陈建华
徐荣
巢玲
XIE Jing;CHEN Jianhua;XU Rong;CHAO Ling(Department of Laboratory,Yichun Vocational and Technical College,Yichun336000,China;Department of Laboratory,the Second Affiliated Hospital of Yichun University,Yichun336000,China;Department of Laboratory,Yichun People's Hospital in Jiangxi Province,Yichun336000,China)
出处
《中国现代医生》
2020年第24期21-23,27,共4页
China Modern Doctor
基金
江西省宜春市科技计划项目(JXYC2015KSA010)。
关键词
抗核抗体
肝自身抗体
乙肝病毒感染
自身免疫性肝病
Antinuclear antibody
Liver autoantibody
Hepatitis B virus infection
Autoimmune liver disease
