摘要
动物药是传统中药的重要组成部分,由于缺乏适宜的研究思路与方法,中药动物药功效物质基础、质量评价研究等不够系统与完善,制约着动物药临床使用、制药工艺和质量控制等方面的应用与发展。为此,本文基于对角类、胶类动物药的研究及认识,结合国内外动物药研究进展与现状,探索性地提出基于"蛋白质/肽组学-修饰组学"研究动物药蛋白质、肽类物质与功效关联规律的思路及方法,即以蛋白质/肽组学的方法系统研究动物药物质组成,并以修饰组学研究蛋白质、肽类成分发生的化学修饰情况与规律,进一步探讨传统功效、成分和修饰组间的规律性与关联性,以期为中药动物药现代化研究和功效物质基础研究等提供借鉴与参考。
Animal derived traditional Chinese medicines(ATCMs)are an important part of traditional Chinese medicine(TCM).The lack of proper ideas and strategies made it not systematic and perfect enough on investigating bioactive components and quality evaluation of ATCMs,which restrict many aspects of ATCMs investigation including clinical applications,pharmaceutical technologies,and quality control.Therefore,based on our previous investigations of animal horn and animal derived gelatin TCMs,and the research progress at home and abroad,ideas and strategies for investigating the correlations between proteins/peptides and their bioactivities in animal horn and animal gelatin derived TCMs based on integrated"proteomics/peptidomics-modifications"methods was proposed.Firstly,proteomics and peptidomics analysis can be used to study proteins and peptides in ATCMs.Secondly,modification analysis can be used to reveal those chemical modifications on proteins and peptides of ATCMs.Thirdly,the correlations between components,modifications and traditional bioactivities can be systemic discussed.Based on the present study,hopefully,enough evidences and reference can be provided to resolve the issues in ATCMs investigations on modernization and bioactive material basis.
作者
刘睿
赵明
段金廒
LIU Rui;ZHAO Ming;DUAN Jin-ao(National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics,Nanjing University of Chinese Medicine,Nanjing 210023,China;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《药学学报》
CAS
CSCD
北大核心
2020年第8期1735-1743,共9页
Acta Pharmaceutica Sinica
基金
国家重点研发计划资助项目(2018YFC1706100)
国家自然科学基金面上项目(81973450)
江苏省高校“青蓝工程”优秀青年骨干教师(2016年)
江苏省“333工程”第三层次(2016年)
中华中医药学会“青年人才托举工程”(QNRC2-C14)
江苏省高等学校自然科学研究项目(19KJB360020)。
关键词
角类
胶类
动物药
蛋白质组学
多肽组学
修饰组学
horn
gelatin
animal derived traditional Chinese medicine
proteomes
peptidomes
modification
