摘要
动物药是传统中药的重要组成部分,其特有功效在中药临床应用中具有不可替代性。鉴于目前动物药研究主要存在化学成分、药效物质基础、毒性物质基础不明确,动物药质量控制体系不健全等关键问题,通过构建基于转录组学-蛋白质组学-多肽组学整合关联分析策略的动物药蛋白多肽类成分研究思路及方法,主要包括基于转录组学的动物药蛋白数据库的构建、动物药蛋白多肽类成分的高分辨质谱分析、蛋白多肽类成分的匹配分析与鉴定以及关联功效的蛋白多肽类成分活性评价等研究内容,试图为动物药蛋白多肽类成分的分析鉴定提供一种切实可行的研究思路及方法,为进一步解决动物药研究存在的关键问题提供技术支持。
Animal medicine is an important part of traditional Chinese medicine,and its unique efficacy is irreplaceable in clinical application.In consideration of the main problems in the research of animal medicine,such as the uncertainty of chemical composition,pharmacodynamic components and toxic components,and the inadequacy of quality control system,this article established a research method of protein and peptides in animal medicine based on transcriptomics-proteomics-peptidomics integrated association analysis strategy,which mainly include the construction of protein database based on transcriptomics,high resolution mass spectrometry analysis,identification and activity evaluation of protein and peptide components.It attempts to provide a practical research method for the analysis and identification of protein and peptide components in animal medicine and technical support for further solving the key issues of animal medicine research.
作者
杨彬
高文远
张艳军
YANG Bin;GAO Wen-yuan;ZHANG Yan-jun(Tianjin State Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,ChinaSchool of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China)
出处
《中草药》
CAS
CSCD
北大核心
2019年第5期1033-1038,共6页
Chinese Traditional and Herbal Drugs
