摘要
为探索桦褐孔菌(Inonotus obliquus)三萜是否能作为α-葡萄糖苷酶/α-淀粉酶天然抑制剂,采用硅胶柱色谱洗脱桦褐孔菌提取物,通过薄层色谱检测进行分离、纯化,结合液质联用色谱和核磁共振波谱对桦褐孔菌中的生物活性物质进行鉴定。结果表明:从桦褐孔菌发酵菌丝体中发现了桦褐孔菌萜D、lawsaritol和白桦脂酸,并从子实体中得到羊毛甾醇、桦褐孔菌醇和栓菌酸;桦褐孔菌子实体中的桦褐孔菌醇对α-葡萄糖苷酶具有最强抑制活性,液体深层发酵产物桦褐孔菌萜D对α-淀粉酶具有最强抑制活性,其IC50值均低于阳性对照的阿卡波糖;酶抑制动力学结果表明,桦褐孔菌醇对α-葡萄糖糖苷酶作用机制为竞争性抑制,桦褐孔菌萜D对α-淀粉酶的抑制类型为非竞争性与竞争性抑制混合。
This study aims to explore whether triterpenoids of Inonotus obliquus are able to be used as natural inhibitors ofα-glucosidase/α-amylase.The extract of Inonotus obliquus was separated and purified by silica gel column chromatography gradient elution and thin layer chromatography,and the bioactive substance of Inonotus obliquus was identified by HPLC and NMR.The results showed that,inoterpene D,lawsaritol,and betulinic acid were found from the fermented mycelium and lanosterol,inotodiol and trametenolic acid were obtained from the sclerotia of Inonotus obliquus.Among them,inotodiol from the sclerotia had the strongest inhibitory activity onα-glucosidase,and inoterpene D from the submerged fermentation product had the strongest inhibitory activity onα-amylase and their IC50 value was lower than that of positive control acarbose.The results of enzyme inhibition kinetics indicated the action mechanism of inotodiol onα-glucosidase was competitive inhibition,and the inhibition type of inoterpene D onα-amylase was mixed non-competitive and competitive inhibition.
作者
黄盼盼
陈程
徐向群
HUANG Panpan;CHEN Cheng;XU Xiangqun(College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China)
出处
《浙江理工大学学报（自然科学版）》
2020年第5期678-686,共9页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
浙江省自然科学基金项目(LY16B020013)。
关键词
桦褐孔菌
三萜
分离
鉴定
Α-葡萄糖苷酶
Α-淀粉酶
Inonotus obliquus
triterpenoids
separation
identification
α-glucosidase
α-amylase
