摘要
在桦褐孔菌(Inonotus obliquus)栽培料中添加茉莉酸甲酯,筛选提高菌核总三萜含量的栽培料和菌株,并研究不同浓度茉莉酸甲酯对桦褐孔菌菌丝生长和菌核形成的影响。结果表明:采用3号栽培料(36%桦树木屑、30%杂木屑,20%玉米芯、10%麦麸、2%大豆粉、1%蔗糖、1%石膏),添加0.92μmoL·L^(-1)茉莉酸甲酯后栽培桦褐孔菌1号菌株,每袋菌核产量(21.9 g)和总三萜含量(70.22 mg·g^(-1))均较高。
Three culture substrates and five strains of Inonotus obliquus were screened for increased triterpenoid content in sclerotium by methyl jasmonate(MeJA)elicitation at 0.92μmoL·L^(-1).Then the effects of MeJA concentration on mycelial growth and sclerotium development of I.obliquus were studied.The results showed that strain No.1 showed a high sclerotium yield of 21.9 g per bag and a high triterpene content of 70.22 mg·g^(-1) using substrate No.3(36%birch sawdust,30%miscellaneous sawdust,20%corncob,10%wheat bran,2%soybean powder,1%sucrose,1%gypsum)supplemented with 0.92μmoL·L^(-1) MeJA.
作者
韩增华
王玉霞
戴肖东
刘佳宁
曲小军
潘钰
高娃
HAN Zenghua;WANG Yuxia;DAI Xiaodong;LIU Jianing;QU Xiaojun;PAN Yu;GAO Wa(Institue of Microbiology,Heilongjiang Academy of Science,Harbin 150010,Heilongjiang,China;Heilongjiang Institute of Atomic Energy,Harbin 150086,Heilongjiang,China)
出处
《食用菌学报》
CSCD
北大核心
2022年第5期65-72,共8页
Acta Edulis Fungi
基金
黑龙江省科学院中试与熟化项目(ZSSH 202005)。
关键词
桦褐孔菌
人工栽培
茉莉酸甲酯
三萜化合物
Inonotus obliquus
artificial cultivation
methyl jasmonate
triterpenoids
