摘要
目的探讨微小RNA-9(miRNA-9)在胃癌细胞株中的表达及其对增殖和迁移的影响。方法运用脂质体转染法将miRNA-9类似物、miRNA-9抑制物和无关序列转染胃癌细胞MKN-45,记为miRNA-9类似物组、miRNA-9抑制物组、阴性对照组,以MKN-45细胞加新鲜的DMEM培养基作为空白对照组,荧光显微镜观察转染效率。转染48 h后,采用CCK-8检测细胞的增殖能力,Transwell小室检测细胞体外迁移行为。结果转染48 h后MKN-45细胞株转染效率达到90%。miRNA-9类似物促进细胞增殖;miRNA-9类似物转染组穿膜细胞数为(1052±71)个,miRNA-9抑制物组为(276±32)个,阴性对照组为(595±42)个,空白对照组为(613±35)个,miRNA-9类似物组穿膜细胞数较空白对照组、阴性对照组增多。结论miRNA-9在胃癌细胞中过表达,与胃癌细胞的恶性生物学行为密切相关。
Objective To investigate the expression of microRNA-9(miRNA-9)in gastric cancer cell lines and its effect on the proliferation and migration of cancer cells.Method The miRNA-9 analogs、miRNA-9 inhibitorsand irrelevent sequence plasmids were transiently transfected into gastric cancer cell lines MKN-45 using the liposome method.Thereafter,all cells were divided into four groups and three groups received plasmids were designated as the miRNA-9 analog group,miRNA-9 inhibitor group,negative control group and MKN-45 cells with fresh DMEM medium were selected as blank control group.The transfection efficiency was observed base on a fluorescence microscope.CCK-8 was used to detect cell proliferation,and Transwell chamber was used to detect the cell migration after 48 hours of transfection.Result The transfection efficiency of MKN-45 cell line was up to 90%at 48 hours of transfection,and miRNA-9 analogues remarkably promote cell proliferation.The count of transmembrane cells in the miRNA-9 analog group was(1052±71),the miRNA-9 inhibitor group was(276±32),the negative control group was(595±42),and the blank control group was(613±35).The number of transmembrane cells in the miRNA-9 analog group was higher than that of either the blank control group or the negative control group.Conclusion Overexpression of miRNA-9 in gastric cancer cells is closely related to the malignant biological behavior of gastric cancer cells.
作者
周建萍
余猛进
毛振彪
ZHOU Jianping;YU Mengjin;MAO Zhenbiao(Department of Emergency Medicine,the First Affiliated Hospital of the Second Military Medical University,Shanghai 200433,China;Department of Gastroenterology,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu,China)
出处
《癌症进展》
2020年第16期1637-1639,1671,共4页
Oncology Progress
关键词
miRNA-9
胃癌细胞
转染
增殖
迁移
miRNA-9
gastric cancer cells
transfection
proliferation
migration
