摘要
目的探讨miR-429是否通过靶向调控SOX2抑制人卵巢癌细胞增殖与促进凋亡,进一步揭示miR-429在卵巢癌中的抑瘤机制。方法收集在南部战区空军医院2015年12月至2017年11月经手术切除的卵巢癌组织58例(相应癌旁正常卵巢组织58例)。将miR-429 mimics转染于卵巢癌SKOV3细胞,以scramble为阴性对照,采用免疫印迹试验检测两组细胞SOX2蛋白的表达。将miR-429 mimics、si-SOX2分别转染于SKOV3细胞中,以scramble为miR-429 mimics的阴性对照,si-control为si-SOX2的阴性对照。采用MTT和凋亡实验检测miR-429mimics与si-SOX2对人卵巢癌细胞增殖与凋亡的影响。结果 qRT-PCR检测结果显示,miR-429在卵巢癌组织的表达水平为0.577±0.088,明显低于正常卵巢组织的1.335±0.218,差异有统计学意义(P<0.05);miR-429在卵巢癌细胞中的表达水平为0.578±0.094,明显低于正常卵巢上皮细胞的1.409±0.230,差异有统计学意义(P<0.05);免疫印迹试验结果显示,miR-429 mimics组SOX2蛋白的表达水平为0.417±0.030,明显低于scramble组的0.782±0.046,差异有显著统计学意义(P<0.01);MTT结果显示,miR-429 mimics组和si-SOX2组细胞的OD值分别为0.474±0.051、0.458±0.042,分别与各自对照组的0.778±0.051、0.734±0.049比较明显减少,差异均有统计学意义(P<0.05);凋亡实验结果显示,miR-429 mimics组和si-SOX2组细胞凋亡率分别为(10.49±0.68)%、(10.62±0.88)%,分别与各自对照组的(6.78±0.57)%、(6.61±0.64)%比较明显增加,差异均有统计学意义(P<0.05)。结论 miR-429可能通过靶向调控SOX2抑制人卵巢癌细胞增殖与促进凋亡。
Objective To investigate whether miR-429 suppresses cell proliferation and promotes apoptosis of human ovarian cancer cells by targeting SOX2, thus to reveal the anti-tumor mechanism of miR-429 in ovarian cancer.Methods From December 2015 to November 2017, 58 cases of ovarian cancer tissue and 58 cases of paracancerous normal ovarian tissue were collected in Air Force Hospital of the Southern Theater Command of the Chinese People’s Liberation Army. miR-429 mimics was transfected into ovarian cancer SKOV3 cells, and scramble was used as a negative control. The expression of SOX2 protein was detected by Western blot. miR-429 mimics and si-SOX2 were transfected into SKOV3 cells, respectively, and scramble was used as the negative control of mi-429 mimics and si-control was used as the negative control of si-SOX2. The effects of miR-429 mimics and si-SOX2 on proliferation and apoptosis of human ovarian cancer cells were detected by MTT and apoptosis assays. Results qRT-PCR showed that the expression of miR-429 in ovarian cancer tissues was 0.577±0.088, significantly lower than 1.335±0.218 in normal ovarian tissues(P<0.05). The expression level of miR-429 in ovarian cancer cells was 0.578 ± 0.094, significantly lower than1.409±0.230 in normal ovarian epithelial cells(P<0.05). Western blot showed that the expression level of SOX2 protein in miR-429 mimics transfected group was 0.417±0.030, significantly lower than 0.782±0.046 in its control group(P<0.01). MTT results showed that the OD values of cells transfected with miR-429 mimics and si-SOX2 group were0.474±0.051, 0.458±0.042, significantly lower than 0.778±0.051, 0.734±0.049 in their control group(P<0.05). Apoptosis assays results showed that the apoptotic rate of cells transfected with mi R-429 mimics and si-SOX2 group were(10.49±0.68)%,(10.62±0.88)%, significantly higher than(6.78±0.57)%,(6.61±0.64)% in the control group(P<0.05). Conclusion miR-429 may inhibit the proliferation and promote apoptosis of human ovarian cancer cells by targeting SOX2.
作者
杨慧芝
谭志琴
万兰
文玉华
林燕蕊
余晓珊
YANGHui-zhi;TAN Zhi-qin;WAN Lan;WEN Yu-hua;LIN Yan-rui;YU Xiao-shan(Department of Gynaecology and Obstetrics,Air Force Hospital of the Southern Theater Command of the Chinese People's Liberation Army, Guangzhou 510000,Guangdong, CHINA)
出处
《海南医学》
CAS
2019年第10期1234-1237,共4页
Hainan Medical Journal
