摘要
目的分析太湖地区宫颈癌及宫颈癌前病变妇女中人乳头瘤病毒(HPV)感染的情况。方法选用聚合酶链式反应(PCR)和基因芯片法对730名宫颈癌和宫颈癌前病变患者进行基因分型。结果宫颈癌前病变和宫颈癌中最常见的基因型为HPV 16型,其在宫颈上皮内瘤样病变I、Ⅱ、Ⅲ级和宫颈癌中的阳性率分别为17.7%(47/266)、24.8%(63/254)、29.5%(39/132)和36.7%(22/60)。HPV检出率最高是宫颈癌患者(86.7%,52/60),并有9种高危HPV型别检出,未见低危型。在宫颈癌患者中优势HPV基因型主要集中于16、18、52、58型,同时宫颈癌患者的感染中75.0%(45/60)为单一高危型别感染;而宫颈癌前病变患者中HPV基因型除HPV 82外所有基因型别均有检出。结论宫颈癌及宫颈癌前病变的发生进展可能与高危HPV多重感染并无关联。HPV16、HPV52、HPV58和HPV18为宫颈癌和宫颈癌前病变的高危亚型。
Objective To investigate the distribution of genotypes among HPV infected women with cervical cancer(CC)and cervical precancerous lesions in the region.Methods 730 patients with cervical cancer and cervical lesions were genotyped by PCR and gene chip.Results The most common genotype in precancerous lesions and cervical cancer was HPV16,and the prevalence rates of HPV infection diagnosed as cervical intraepithelial lesions(CIN)I,II,III and CC were 17.7%(47/266),24.8%(63/254),29.5%(39/132)and 36.7%(22/60),respectively.HPV detection rate was the highest in cervical cancer patients(86.7%,52/60),with nine high-risk types detected and no low-risk types in the group.The dominant HPV genotypes in cervical cancer patients were mainly type 16,18,52,58 with single high-risk HPV infection significantly higher than double infection and multiple infection,accounting for 75.0%(45/60).All HPV genotypes except for HPV82 were detected in patients with cervical precancerous lesions.Conclusion The progression of cervical cancer and cervical precancerous lesions may not be associated with multiple HPV infections.HPV16,HPV52,HPV58 and HPV18 are high risk subtypes of cervical cancer and cervical precancerous lesions.
作者
陆斐斐
陈旭
李琼
陆静芬
LU Feifei;CHEN Xu;LI Qiong;LU Jingfen(Clinical laboratory of Suzhou Ninth People’s Hospital,Jiangsu Province,215200,China)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2020年第8期857-859,共3页
Chinese Journal of Aids & STD
基金
苏州市吴江区社会发展科技计划项目(WS201302)。
