摘要
采用双水相萃取法辅助醇提法来提取纯化杜仲(Eucommia ulmoides Oliver)中的总黄酮和绿原酸。通过单因素试验和Box-Behnken响应面法确定最佳提取工艺,利用乙醇/无机盐双水相体系将杜仲提取物中的总黄酮和绿原酸进行有效分离。结果表明,在最佳提取条件下,杜仲总黄酮的最佳双水相萃取体系为乙醇的质量分数50%、K2HPO4的质量分数20%,此时,相比R为2.93,分配比D为10.35,杜仲总黄酮的萃取率达77.94%。绿原酸的最佳双水相萃取体系为乙醇的质量分数60%、K2HPO4的质量分数20%,此时,相比R为3.43,分配比D为12.32,杜仲绿原酸的萃取率达78.22%。开发条件温和、成相稳定、用时较短的双水相萃取技术,对杜仲的开发利用有重要意义。
In this study,the two-phase extraction method was used to assist the extraction of total flavonoids and chlorogenic acid in Eucommia ulmoides Oliver.The optimal extraction process was determined by single factor and Box-Behnken response surface method test,and the total flavonoids and chlorogenic acid in Eucommia ulmoides extract were separated by ethanol/inorganic salt aqueous twophase system.The results showed that under the optimal extraction conditions,the best aqueous two-phase extraction system for total flavonoids in Eucommia ulmoides was 50%ethanol content with 20%mass fraction of K2HPO4,the phase ratio R was 2.93 and the dis⁃tribution ratio D was 10.35,and the extraction ratio could reach 77.94%.The best aqueous two-phase extraction system for chlorogen⁃ic acid was 60%ethanol with 20%K2HPO4 in water,the phase ratio R was 3.43 and the distribution ratio D was 12.32 with the extrac⁃tion ratio up to 78.22%.The dual aqueous phase extraction technology with mild development conditions,stable phase and short time is great significant for the development and utilization of Eucommia ulmoides.
作者
张亦琳
延永
张琴
ZHANG Yi-lin;YAN Yong;ZHANG Qin(College of Biology Pharmacy and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China)
出处
《湖北农业科学》
2020年第13期127-131,共5页
Hubei Agricultural Sciences
基金
陕西省自然科学基础研究计划项目(2018JQ2070)
陕西省教育厅专项科研计划项目(18JK0250)
商洛学院基础研究项目(116SKY021)。
