摘要
为开发利用辣椒籽资源,以辣椒籽为原料,研究高压脱脂辣椒籽分离蛋白的提取工艺及其功能特性。以辣椒籽分离蛋白提取量为考察指标,在单因素实验基础上,研究pH、料液比、提取时间和提取温度对辣椒籽分离蛋白提取量的影响,采用响应面优化法确定最佳提取工艺。结果表明,高压脱脂辣椒籽分离蛋白的最佳提取条件为pH 9.5、料液比1∶39、37℃、38min,提取量达6.27g/100g,等电点(PI)为4,纯度为90.29%。高压脱脂辣椒籽分离蛋白的持水力(3.57±0.12)g/g和持油力(3.15±0.17)g/g较溶剂脱脂辣椒籽分离蛋白分别提高了46.31%和34.04%,说明高压脱脂分离蛋白具有较好的功能特性。
In order to develop and utilize the resources of Capsicumseeds,the extraction technology and functional properties of high-pressure defatted Capsicum seeds protein isolate are studied using Capsicumseeds as the materials.Based on the single factor experiment,the extraction yield of Capsicumseeds protein isolate is taken as the index,the effects of pH values,ratios of solid to liquid,extraction time and extraction temperatures on the extraction yield of Capsicumseeds protein isolate are studied,and the optimal extraction process is determined by response surface methodology.The results show that the optimum extraction conditions are pH 9.5,solid-liquid ratio 1∶39,37℃,38min,the extraction yield is 6.27g/100g,isoelectric point(PI)is 4,and the purity is,and the purity 90.29%.The water holding capacity(3.57±0.12)g/g and oi l holding capacity(3.15±0.17)g/g of higdefatted Capsicum seeds protein isolate Capsicum seeds protein isolate,indicating that hig is 46.31%and 34.04%high erthan that o f solvent degreased h-pressure degreased protein isolate has better functional properties
作者
马燕
孟伊娜
邹淑萍
张谦
廖小军
赵靓
MA Yan;MENG Yi-na;ZOU Shu-ping;ZHANG Qian;LIAO Xiao-jun;ZHAO Liang(Institute of Agro-products Storage and Processing,Xinjiang Academy of Agricultural Sciences,Xinjiang Deep Processing and Engineering Technology Research Center of Main Byproducts,Urumqi 830091,China;National Engineering Technology Research Center for Fruit and Vegetable Processing,Key Open Laboratory of Fruit and Vegetable Processing,Ministry of Agriculture and Rural Affairs,Beijing Key Laboratory of Food Non-thermal Processing,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China)
出处
《中国调味品》
CAS
北大核心
2020年第8期54-60,共7页
China Condiment
基金
新疆维吾尔自治区自然科学基金资助项目“超高压对辣椒籽蛋白特性的影响及其机理研究”(2016D01B031)。
关键词
高压脱脂
辣椒籽分离蛋白
响应面优化
功能特性
high-pressure defatted
Capsicum seeds protein isolate
response surface methodology
functional properties
