摘要
【目的】性信息素受体(sex pheromone receptors,PRs)是雄蜂感受蜂王上颚腺信息素(queen mandibular pheromone,QMP)的重要受体。本研究分析中华蜜蜂Apis cerana cerana(简称“中蜂”)和意大利蜜蜂Apis mellifera ligustica(简称“意蜂”)雄蜂触角和大脑中候选性信息素受体基因Pr s受QMP刺激下的表达特征,为探索蜜蜂气味受体(OR)基因的功能研究提供理论依据。【方法】利用qRT-PCR技术检测分析分别用10μL QMP[7.04μg/μL反式-9-氧代-2-癸烯酸(9-ODA)+1.26μg/μL 9-羟基-2-癸烯酸(9-HDA)+0.03μg/μL对羟基苯甲酸甲酯(HOB)]和10μL 7.04μg/μL 9-ODA处理对飞行状态和爬行状态下的中蜂雄蜂和意蜂雄蜂触角和大脑中4个气味受体基因(Or10,Or11,Or 18和Or 170)的mRNA表达量的影响。【结果】与空白对照组相比,QMP及9-ODA均能显著下调中蜂雄蜂和意蜂雄蜂触角与大脑中Or 11的mRNA表达量;中蜂雄蜂触角中AcOr18和AcOr 170基因受QMP及9-ODA刺激后mRNA表达量显著下调;QMP与9-ODA均能显著降低中蜂雄蜂和意蜂雄蜂大脑中Or 170的mRNA表达量。在QMP或9-ODA刺激下,飞行中蜂雄蜂大脑中AcOr 11的mRNA表达量显著高于爬行中蜂雄蜂大脑中的,而飞行与爬行中蜂雄蜂触角中AcOr 11的mRNA表达量没有显著差异。【结论】中蜂和意蜂雄蜂触角与大脑中气味受体基因Or 11均能应答蜂王上颚腺信息素9-ODA,且受9-ODA刺激后Or 11的mRNA表达下调。
【Aim】Sex pheromone receptors(PRs)are important receptors for the perception of queen mandibular pheromone(QMP)in honeybee drones.The aim of this study is to analyze the expression characteristics of candidate sex pheromone receptor genes in the antennae and brains of Apis cerana cerana and Apis mellifera ligustica drones subjected to QMP stimulation,so as to provide the theoretical basis for further studies on the biological function of odorant receptor(OR)genes in honeybees.【Methods】qRT-PCR was employed to detect and analyze the mRNA expression levels of four OR genes including Or10,Or11,Or 18 and Or 170 in the antennae and brains of flying and crawling drones of A.c.cerana and A.m.ligustica exposed to 10μL QMP[7.04μg/μL(E)-9-oxodec-2-enoic acid(9-ODA)+1.26μg/μL 9-hydroxydec-2-enoic acid(9-HDA)+0.03μg/μL methylp-hydroxybenzoate(HOB)]and 10μL 7.04μg/μL 9-ODA,respectively.【Results】The results showed that QMP and 9-ODA significantly inhibited the transcription of Or 11 in the antennae and brains of A.c.cerana and A.m.ligustica drones compared to the blank control group.The mRNA expression levels of AcOr 18 and AcOr 170 in the antennae of A.c.cerana drones were down-regulated significantly by QMP and 9-ODA.Moreover,QMP and 9-ODA significantly decreased the mRNA expression levels of Or 170 in the brains of drones of the two bees.After A.c.cerana drones were subjected to the stimulation of QMP or 9-ODA,the mRNA expression level of AcOr 11 was significantly higher in the brain of flying drones than in the brain of crawling drones,whereas showed no significant difference in the antennae between flying drones and crawling drones.【Conclusion】Or 11 in the antennae and brains of A.c.cerana and A.m.ligustica drones can respond to 9-ODA stimulation,and its expression is down-regulated after 9-ODA stimulation.
作者
刘俊峰
李茫
王子龙
何旭江
曾志将
LIU Jun-Feng;LI Mang;WANG Zi-Long;HE Xu-Jiang;ZENG Zhi-Jiang(Honeybee Research Institute,Jiangxi Agricultural University,Nanchang 330045,China;Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2020年第6期697-707,共11页
Acta Entomologica Sinica
基金
国家自然科学基金项目(31572469)
国家蜂产业技术体系(CARS-44-kxj15)。
关键词
中华蜜蜂
意大利蜜蜂
雄蜂
蜂王上颚腺信息素
气味受体
Apis cerana cerana
Apis mellifera ligustica
drone
queen mandibular pheromone
odorant receptor
