摘要
为研究精胺对发育早期雌鹅卵巢生殖激素受体基因表达的影响,本实验分别用禽用生理盐水和5 mg/kg体重的精胺给28日龄雏鹅连续灌胃2周后称重,采集血液和卵巢组织,统计平均日增重、卵巢指数,并利用实时荧光定量PCR检测鹅下丘脑、垂体和卵巢组织中FSHR、LHR、ER1和ER2表达量。结果表明:5 mg/kg体重的精胺灌胃对鹅体重、卵巢重和卵巢/体重比均无显著影响;灌胃精胺组雏鹅卵巢组织LHR和ER2表达量分别是对照组的4.78倍和1.65倍(P<0.05)。上述结果表明,外源性精胺可促进雏鹅卵巢组织ER2和LHR的表达,提示精胺能够通过介导生殖激素受体表达影响鹅的早期卵巢发育。
To study the effect of spermine on reproductive hormone receptor expression in ovary tissues of geese during early development stage, geese aged 28 day-old were fed with spermine(per os) about 2 weeks. The mRNA expression levels of FSHR, LHR, ER1 and ER2 in ovary tissues were examined by real-time PCR. The results showed that the difference in body weight, ovary weight and the ratio of ovary/body weight between 5 mg/kg spermine-treated geese and the control group was not observed(P>0.05). The mRNA expression levels of LHR and ER2 in the ovary of spermine-treated geese was 4.78-and 1.65-fold higher compared with the control group, respectively(P<0.05). In conclusion, spermine upregulates ER2 and LHR transcriptions in ovarian tissues. These results indicate that spermine mediates ovarian early development through mediating hormone receptor gene expressions.
作者
易治鑫
李林祥
徐进
李苗
姜冬梅
YI Zhixin;LI Linxiang;XU Jin;LI Miao;JIANG Dongmei(College of Animal Science and Technology,Sichuan Agricultural University,Sichuan Chengdu 611130,China;Bazhong Academy of Agriculture and Forestry Sciences,Sichuan Bazhong 636000,China)
出处
《中国畜牧杂志》
CAS
北大核心
2020年第5期92-94,99,共4页
Chinese Journal of Animal Science
基金
国家自然科学基金项目(31872358)
四川省教育厅青年基金项目(18ZB0469)。
关键词
雏鹅
精胺
卵巢
激素受体
Stating geese
Spermine
Ovary
Hormone receptor
