摘要
【目的】信息素是个体之间传递信息的重要分子,研究性信息素对斜纹夜蛾Spodoptera litura嗅觉相关基因表达的影响对于增加性信息素作用机理的认识及其应用有重要的意义。【方法】本研究通过实时定量PCR(qRTPCR)技术探究在性信息素刺激处理条件下,斜纹夜蛾成虫嗅觉相关基因abp,pbp和or表达水平的变化;利用性信息素在田间诱捕斜纹夜蛾雄蛾,并通过自动计数器记录每小时诱虫量,从而间接显示其交配行为的节律性。【结果】斜纹夜蛾雄虫触角中嗅觉相关基因abp,pbp和or的表达具有节律特性。经性信息素化合物(Z9,Z11-14:OAc+Z9,Z12-14:OAc)刺激处理后,abp,pbp和or表达量也发生了显著的改变。通过记录田间性信息素诱捕器在一天中不同时间段内诱捕的雄蛾数量,发现诱捕到的斜纹夜蛾也具有节律特性。【结论】基因表达水平上的节律特性可能与雄虫交配活动的节律相关联,说明性信息素处理也在一定程度上改变了其节律及其对性信息素的神经反应。这一结果也首次从基因水平证明性信息素的刺激处理提高了周缘神经系统对性信息素反应的敏感性,有助于我们理解性信息素作用的分子机理,对迷向及性诱和测报应用具有指导意义。
【Aim】Insect pheromones are the chemical molecules for communication between individuals of insects. To better understand their neurological mechanisms and make better application, it is important to study the effect of sex pheromone on the expression of olfactory genes. 【Methods 】The quantitative real-time PCR（ qRT-PCR） was used to study the changes in the expression levels of olfactory genes abp,pbp and or in Spodoptera litura male moths after exposure to synthetic sex pheromone. A newly developed pheromone-based electronic counting system was adopted to monitor the population dynamics of S. litura by sex pheromone trapping. 【Results】The expression of abp,pbp and or showed circadian rhythm in the antennae of male moths of S. litura. The expression levels of abp,pbp and or and their circadian rhythm were significantly affected by the exposure to synthetic sex pheromone（ Z9,Z11-14： OAc ＋ Z9,Z12-14： OAc）. The population dynamics of S. litura male moths monitored by sex pheromone trapping also showed clear circadian rhythm. 【Conclusion】The circadian rhythm of the gene expression was correlated with that of the male mating behavior. Our results provided the first evidence to elucidate the enhancement of peripheral and central neuronal sensitivity of olfactory system of S. litura moths stimulated by sex pheromone at the gene expression level by the exposure of pheromone compounds. These results would be helpful to our understanding of the molecular mechanisms of pheromone recognition,the application of insect pheromone by mating disruption and pest forecasting andcontrol by mass trapping with sex pheromone.
出处
《昆虫学报》
CAS
CSCD
北大核心
2015年第3期237-243,共7页
Acta Entomologica Sinica
基金
公益性行业(农业)科研专项(201203036)
宁波市重大科技项目(2012C10026)
关键词
斜纹夜蛾
性信息素
信息素结合蛋白
气味受体
节律
基因表达
Spodoptera litura
sex pheromone
pheromone binding protein
olfactory receptor
circadian rhythm
gene expression
