摘要
目的明确甘松乙酸乙酯提取物(EFNC)对高原缺氧诱导脑组织损伤的保护作用。方法建立低压低氧诱导小鼠脑组织损伤模型,经EFNC预处理后,利用相关试剂盒检测脑组织中氧化应激和能量代谢相关指标,利用Western blot技术检测脑组织相关蛋白的表达。结果给予EFNC预处理后,能够减少低压低氧小鼠脑组织中H2O2和MDA含量,增加GSH和ATP的水平,提高SOD、CAT、GSH-Px和ATP酶的活力以及Nrf2和HO-1的表达。结论EFNC对高原缺氧诱导脑组织损伤具有保护作用,其作用机制可能与激活Nrf2-HO-1信号通路,提高抗氧化能力以及改善能量代谢障碍有关。
Objective To identify the ptotective effect of ethyl acetate extract fraction of Nardostachys chinensis Batal.(EFNC)against high altitude hypoxia-induced brain injury.Methods Establishment of hypobaric hypoxia induced brain injury model in mice,after administration of EFNC,the index related to oxidative stress and energy metabolism in brain tissue are measured using commercial assay kit.The expression of related proteins in brain tissue is detected by western blot analysis.Results Prior administration of EFNC reduced the level of hydrogen peroxide(H2O2)and malondialdehyde(MDA),elevated the glutathione(GSH)and triphosphate(ATP)content and activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)and ATPase as well as further increased the expression of Nrf2 and HO-1 in brain of mice under hypobaric hypoxia condition.Conclusion EFNC has a preventive effect on brain injury induced by HH,which may be associated with activation of Nrf2/HO-1 pathway,improvement of antioxidant activity and amelioration of metabolic disorders.
作者
孙杨
魏崇莉
赵彤
马慧萍
景临林
SUN Yang;WEI Chong-li;ZHAO Tong;MA Hui-ping;JING Lin-lin(First Outpatient Department,the 940 th Hospital of Joint Logistics Support force of PLA,Lanzhou 730050,China;Department of Pharmacy,the 940 th Hospital of Joint Logistics Support force of PLA,Lanzhou 730050,China)
出处
《现代中药研究与实践》
CAS
2020年第3期13-17,共5页
Research and Practice on Chinese Medicines
基金
国家自然科学基金资助项目(81872796)
军队后勤科研计划(CWH17J010)
中国博士后科学基金(2012M521926)。
关键词
甘松
乙酸乙酯提取物
高原缺氧
脑组织损伤
氧化应激
Nardostachys chinensis Batal.
ethyl acetate extract fraction
high altitude hypoxia
brain injury
oxidative stress
