摘要
噬菌体在维持宿主肠道微环境中起着重要作用.运用宏基因组学方法全面探究大熊猫肠道噬菌体的多样性.共注释到548种噬菌体,主要来自11个科38个属548个种.大多数噬菌体主要来自长尾病毒科Siphoviridae、肌尾噬菌体科Myoviridae和短尾噬菌体科Podoviridae,所占比例分别为41.7%、27.6%和19.5%.其中未分类到科的噬菌体占10.5%.38个属主要为λ样病毒属(10.3%)、T4样病毒属(8.0%)、P1样病毒属(7.1%)、Phieco32样病毒属(5.3%)、T5样噬菌体属(3.4%)等.548个噬菌体中有22种丰度超过了1%,主要为肠细菌噬菌体P1(7.0%)、链球菌噬菌体LYGO9(6.1%)、肠细菌噬菌体λ(5.3%)、肠细菌噬菌体Phieco32(5.2%)、链球菌噬菌体phi30c(3.8%)等.此外,不同大熊猫繁育基地之间噬菌体群落结构存在显著差异(科水平:R=0.168,P<0.01;属水平:R=0.128,P<0.01;种水平:R=0.291,P<0.01),在α多样性指数方面SSP基地的Chao1指数与其他两个基地之间存在着显著差异.本研究发现大熊猫肠道噬菌体种群丰富多样,且因生境不同而显著变化;结果有助于挖掘大熊猫肠道噬菌体在疾病防控及治疗的潜力,为保障大熊猫的肠道健康提供新的思路.
Bacteriophages play an important role in maintaining the intestinal microenvironment of the host.Currently,only a few studies have reported on the intestinal bacteriophages of giant pandas.In this study,we explored the intestinal bacteriophages of giant pandas based on metagenomics.We found a total of 548 kinds of bacteriophages,mainly belonging to 11 families,38 genera,and 548 species.Most of the bacteriophages were primarily from Siphoviridae,Myoviridae,and Podoviridae,accounting for 41.7%,27.6%,and 19.5%,respectively,of the total population.Among them,10.5%were bacteriophages that were not classified into families yet.The 38 identified genera mainly included lambda-like viruses(10.3%),T4-like viruses(8.0%),P1-like viruses(7.1%),Phieco32-like viruses(5.3%),and T5-like viruses(3.4%).Among the 548 species of phages,22,accounting for more than 1%,mainly included enterobacterial bacteriophage P1(7.0%),streptococcal bacteriophage LYGO9(6.1%),enterobacterial bacteriophage lambda(5.3%),enterobacterial bacteriophage Phieco32(5.2%),and streptococcal bacteriophage phi30c(3.8%).Moreover,there were significant differences in the structure of bacteriophage community among different breeding bases of giant pandas(family level:R=0.168,P<0.01;genus level:R=0.128,P<0.01;species level:R=0.291,P<0.01).There were significant differences in alpha diversity index between the SSP base and other two bases.This study reveals that the intestinal phages in giant pandas are of rich diversity and varied with different habitats,which would help to further address the potential of intestinal bacteriophages in disease prevention,control,and treatment and provide new insights into gut health protection of giant pandas.
作者
赵思越
李才武
杨盛智
李果
何永果
吴代福
黄炎
张和民
邹立扣
ZHAO Siyue;LI Caiwu;YANG Shengzhi;LI Guo;HE Yongguo;WU Daifu;HUANG Yan;ZHANG Heming;ZOU Likou(Faculty of Applied Microbiology,College of Resources,Sichuan Agricultural University,Chengdu 611130,China;Key Laboratory of State Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park,China Conservation and Research Center for the Giant Panda,Dujiangyan 611830,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第3期489-498,共10页
Chinese Journal of Applied and Environmental Biology
基金
大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室开放基金项目(中国大熊猫保护研究中心,KLSFGAGP2020.003)
国家自然科学基金项目(31400066)资助。
