摘要
铜绿假单胞菌是孵化过程中造成腐蛋的主要病原菌,拟尝试用裂解性噬菌体防治。选用腐蛋中筛选的铜绿假单胞菌PA-MH12,以此为宿主从河水中筛选到1株裂解性噬菌体MH12-Q,对其进行了生物学特性研究,体外进行了对铜绿假单胞菌所致腐蛋的防控效果试验。结果显示:噬菌体MH12-Q属于肌尾噬菌体科(Myoviridae),效价可达到1013pfu·mL-1,完全裂解效价(CLC)为1010pfu·mL-1,最适生长温度为37℃,pH稳定性好,最适pH为6~8,且其在pH4~10的效价都能维持在较高的水平。最佳感染复数(OMOI)为0.001,感染宿主的潜伏期为30 min,爆发期为70 min。噬菌体MH12-Q可使高浓度铜绿假单胞菌的感染率从70%降低到40%。铜绿假单胞菌噬菌体MH12-Q的生物学性质适合生产应用,对孵化场腐蛋具有较好的防控作用,能够提高感染铜绿假单胞菌的鸡胚成活率。
Pseudomonas aeruginosa is the main pathogen causing rotten eggs during incubation,we attempted to control it with lytic phage.In this work,a strain of virulent phage MH12-Q was sifted from the river water sample by using Pseudomonas aeruginosa PA-MH12 isolated from the rotten eggs.The biological characteristics of the phage were studied and its prevention and control effect on Pseudomonas aeruginosa induced rotten egg were tested.The phage MH12-Q was sequenced and identified as Myoviridae.The titer of phage MH12-Q can reach 1013pfu·mL-1,the complete lysis concentration(CLC)of phage MH12-Q is 1010pfu·mL-1,and the optimal growth temperature is 37℃.The optimum pH is 6-8 for the stability of the phage and its titer can maintain a high level at pH 4-10.Its optimal multiplicity of infection(OMOI)is 0.001,the incubation period of infected hosts is 30 min,and the outbreak period is 70 min.The phage with the biological properties above can reduce the infection rate of high concentration Pseudomonas aeruginosa from 70%to 40%.The biological properties of Pseudomonas aeruginosa phage MH12-Q are suitable for production and application,which has a good prevention and control effect on rotten eggs in hatchery and can improve the survival rate of chicken embryos infected with Pseudomonas aeruginosa.
作者
刘晓文
张庆
宋新慧
骆延波
胡明
李璐璐
齐静
张印
赵效南
刘玉庆
LIU Xiaowen;ZHANG Qing;SONG Xinhui;LUO Yanbo;HU Ming;LI Lulu;QI Jing;ZHANG Yin;ZHAO Xiaonan;LIU Yuqing(School of Life Sciences, Shandong Normal University, Jinan 250014, China;Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences,Jinan 250100, China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2020年第7期1756-1763,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家重点研发计划(2018YFD0500500,2016YFD0501300)
山东省重大科技创新工程(2019JZZY010719)
山东省农业科学院农业科技创新工程(GXGC2017A01,CXG2018E10)
国家科技重大专项(2018ZX10733402-013)
传染病预防控制国家重点实验室开放课题(2019SKLID317)。
关键词
铜绿假单胞菌
腐蛋
裂解性噬菌体
生物学性质
防治
Pseudomonas aeruginosa
rotten eggs
lytic phage
biological property
prevention and control
