摘要
细菌在烟叶发酵过程中起着重要作用,为探索烟叶的发酵机理,采用Illumina miseq高通量测序技术（Highthroughput sequencing,HTS）和传统微生物分离方法,对墨西哥不同发酵时期雪茄外包皮表面细菌群落结构、丰富度及演替进行研究.（1）HTS获得有效序列条数514 641条,包含65个OTUs,主要种属为棒状杆菌属（Corynebacterium）、葡萄球菌属（Staphylococcus）、不动杆菌属（Acinetobacter）、假单胞菌属（Pseudomonas）、芽孢杆菌属（Bacillus）等.HTS结果表明,墨西哥雪茄外包皮表面细菌群落组成丰富,细菌群落结构随发酵进程而变化,优势微生物由棒状杆菌属（44.64%）和假单胞菌属（40.88%）演替为后期的葡萄球菌属（72.78%）.α多样性结果表明FJQY-1和FJQY-2样品的细菌群落具有较高的丰富度.β多样性分析发现FJQY-2、FJQY-3和FJQY-4三个样品细菌结构及丰度较为相似.（2）传统分离结果显示,不同发酵时期雪茄外包皮表面细菌主要为芽孢杆菌属（55.10%）和葡萄球菌属（22.45%）,与HTS结果比较,单一传统分离方法不能全面反映不同时期雪茄外包皮表面细菌群落结构和演替.本研究表明,在不同发酵时期,雪茄外包皮表面细菌发生着群落演替;HTS测序在揭示微生物群落演替中发挥了重要作用;传统微生物分离鉴定方法不能全面反映微生物多样性及群落演替,但能获得发酵过程中的部分微生物用于后期功能研究.
Bacteria play an important role in the process of cigar fermentation. It is important to study the succession of the bacterial community during cigar fermentation to understand the mechanism of tobacco fermentation. This experiment analyzed the community structure of surface bacteria, species richness, and the succession of bacteria on a Mexican cigar wrapper at different fermentation stages using Illumina Mi Seq high-throughput sequencing technology（HTS） and traditional isolation. The HTS obtained 514 641 effective sequences, including 65 operational taxonomic units（OTUs）. The main microbial species included Corynebacterium sp., Staphylococcus sp., Acinetobacter sp., Pseudomonas sp., and Bacillus sp. The results of the HTS showed an abundant surface bacterial community, and the structure of the community changed with the fermentation process. The dominant microorganism genera were Corynebacterium（44.64%）, Pseudomonas（40.88%）, and Staphylococcus（72.78%）. The FJQY-1 and FJQY-2 bacterial communities had higher alpha diversity richness than that of the other samples. The FJQY-2, FJQY-3, and FJQY-4 samples had similar beta diversity values that were high. Traditional isolation showed that the dominant bacteria during fermentation were Bacillus sp.（55.10%） and Staphylococcus sp.（22.45%）, which does not comprehensively reflect the bacterial community and succession on the cigar wrapper at different periods. There was succession of the bacterial community during the fermentation process. The HTS plays a vital role in revealing the succession of the bacterial community. Traditional isolation and identification cannot fully ref lect the diversity and succession of the bacterial community, however, it can be helpful in future research on microbial function during the fermentation process.
作者
张鸽
梁开朝
辛玉华
王娟
李书贵
李爱军
王帆
张海波
刘好宝
ZHANG Ge;LIANG Kaichao;XIN Yuhua;WANG Juan;LI Shugui;LI Aijun;WANG Fan;ZHANG Haibo;LIU Haobao(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Haikou Cigar Research Institute,Hainan Provincial Branch of China National Tobacco Corporation,Haikou 571100,China;Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China;Qingdao Agricultural University,Qingdao 266109,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2018年第4期783-788,共6页
Chinese Journal of Applied and Environmental Biology
基金
海南省科技计划项目(ZDYF2017155)
中国烟草总公司海南省公司科技重点项目(201746000024052,201746000024056)资助~~
关键词
雪茄外包皮
高通量测序
发酵时期
细菌多样性
群落演替
cigar wrapper
high throughout sequencing
fermentation stage
bacteria diversity
community succession
