摘要
目的:探讨川芎嗪对大鼠脑缺血再灌注损伤的作用及机制。方法:采用线栓法建立大鼠脑缺血再灌注损伤模型。评估川芎嗪对大鼠脑梗死范围、脑含水量、神经功能症状及脑组织病理形态,以及大鼠血清和脑组织IL-6、TNF-α和MCP-1表达的影响。采用Western Blot检测大鼠脑组织VEGF、PI3K、P-AKT及AKT的蛋白表达水平。结果:川芎嗪对脑缺血再灌注损伤有保护作用,其可以减轻大鼠脑梗死体积、脑含水量、神经功能症状及病理形态(P<0.05),此外,其能够下调促炎细胞因子IL-6、TNF-α和MCP-1的表达(P<0.05),增加脑组织VEGF、PI3K、P-AKT/AKT的表达(P<0.05)。结论:川芎嗪预处理对脑缺血再灌注具有明显的保护作用,且该作用可能与激活VEGF介导PI3K-AKT信号通路有关。
Objective:To investigate the effect and mechanism of tetramethylpyrazine on cerebral ischemia-reperfusion injury in rats.Methods:A rat model of cerebral ischemia-reperfusion injury was established by suture-occluded method.The effects of tetramethylpyrazine on the infarct size,brain water content,neurological symptoms and pathological morphology of brain,as well as the expression of IL-6,TNF-αand MCP-1 in serum and brain tissue of rats were evaluated.The protein expression levels of VEGF,PI3K,P-AKT and AKT in rat brain were detected by Western Blot.Results:Tetramethylpyrazine has protective effects on cerebral ischemia-reperfusion injury,which can alleviate cerebral infarct size,brain water content,neurological symptoms and pathological morphology(P<0.05).In addition,it can down-regulate the expression of pro-inflammatory cytokines IL-6,TNF-αand MCP-1 and increase the expression of VEGF,PI3K and P-AKT/AKT in brain tissue(P<0.05).Conclusion:Tetramethylpyrazine pretreatment has a protective effect on cerebral ischemia-reperfusion,which may be related to the activation of VEGF-mediated PI3K-AKT signaling pathway.
作者
朱百科
王新新
赵恒
ZHU Baike(The Third Hospital of Shandong Province,Shandong Ji'nan 250031,China)
出处
《河北医学》
CAS
2020年第6期912-917,共6页
Hebei Medicine
基金
新疆维吾尔自治区自然科学基金,(编号:2017D01C177)。
关键词
脑缺血
再灌注损伤
川芎嗪
Cerebral ischemia
Reperfusion injury
Tetramethylpyrazine
