摘要
目的探讨二苯乙烯苷(TSG)对大鼠脑缺血再灌注损伤Nalp3、Caspse-1、白介素-18(IL-18)表达的影响。方法选取60只SD大鼠随机分成5组,即假手术组、模型组、TSG低剂量组、TSG中剂量组、TSG高剂量组,每组12只大鼠。夹闭双侧颈总动脉缺血2 h,取下动脉夹恢复血流灌注制备脑缺血再灌注损伤动物模型,假手术组麻醉及分离双侧颈总动脉,不夹闭双侧颈总动脉。TSG低、中、高剂量组分别给予TSG 30、60、90 mg/kg,每天一次腹腔注射给药,连续给药7 d,假手术组、模型组均给予等剂量的生理盐水。术后第8天,麻醉大鼠,留取实验相关标本备用。用光学显微镜观察HE染色的海马组织形态学变化;采用干湿重法计算各组大鼠大脑的含水量;用RT-PCR法检测各组大鼠海马Nalp3 mRNA、Caspase-1 mRNA、IL-18 mRNA的表达。结果HE染色结果显示,模型组大鼠海马区神经细胞损伤严重,TSG中、高剂量组大鼠海马神经损伤减轻。模型组大鼠脑组织含水量以及海马Nalp3 mRNA、Caspase-1 mRNA、IL-18 mRNA表达高于假手术组,差异有统计学意义(P<0.05);TSG中、高剂量组大鼠脑组织含水量及海马Nalp3 mRNA、Caspase-1 mRNA、IL-18 mRNA表达低于模型组,差异有统计学意义(P<0.05)。结论TSG可通过抑制脑缺血再灌注损伤大鼠海马组织Nalp3、Caspse-1、IL-18的表达,减轻炎症级联发应,从而发挥对神经细胞的保护作用。
Objective To explore the effect of tetrahydroxystilbene glucoside(TSG)on the expression of Nalp3,Caspse-1,interleukin-18(IL-18)in rats with cerebral ischemia-reperfusion injury.Methods Sixty SD rats were randomly divided into five groups,namely sham operation group,model group,TSG low-dose group,TSG medium-dose group,TSG high-dose group,with 12 rats in each group.The bilateral common carotid arteries were clamped for 2 h,and the arterial clamps were removed to restore blood perfusion to prepare the animal model of cerebral ischemia-reperfusion injury.The sham operation group was anesthetized and bilateral common carotid arteries were separated without clamping.TSG low,medium and high dose groups were given TSG 30,60,90 mg/kg,intraperitoneal injection once a day for 7 consecutive days.The sham operation group and model group were given the same dose of normal saline.On the 8th day after the operation,the rats were anesthetized,and the related specimens were reserved.The histomorphological changes of HE staining hippocampus were observed by light microscope.The brain water content of rats in each group was calculated by dry and wet weight method.The expressions of Nalp3 mRNA,Caspase-1 mRNA and IL-18 mRNA in hippocampus were detected by RT-PCR.Results HE staining showed that the hippocampal nerve cells in the model group were seriously damaged.The damage of hippocampal nerve cells in TSG medium and high dose group were significantly reduced.The brain water content of rats in the model group and the expressions of Nalp3 mRNA,Caspase-1 mRNA and IL-18 mRNA in the hippocampus were higher than those in the sham operation group,and the differences were statistically significant(P<0.05).The brain water content and the expressions of Nalp3 mRNA,Caspase-1 mRNA and IL-18 mRNA in the hippocampus of rats in the middle and high-dose TSG groups were lower than those in the model group,and the differences were statistically significant(P<0.05).Conclusion TSG can inhibit the expression of Nalp3,Caspse-1,and IL-18 in the hippocampus
作者
陈雪
张嫄怡
段文彪
CHEN Xue;ZHANG Yuanyi;DUAN Wenbiao(Department of Pathology,Zhaoqing Medical College,Guangdong Province,Zhaoqing526020,China;School Office,Zhaoqing Medical College,Guangdong Province,Zhaoqing526020,China)
出处
《中国当代医药》
CAS
2022年第13期10-13,19,F0003,共6页
China Modern Medicine
基金
广东省肇庆市科技创新指导类项目(2021040315010)
肇庆医学高等专科学校中青年科技基金项目(Zqyq19-003)。
