摘要
目的:建立离子色谱法(IC)测定26种中药材二氧化硫(SO2)残留量的检测方法。方法:样品经加酸水蒸气蒸馏提取,3%过氧化氢溶液作为吸收液,将SO2氧化为硫酸根(SO42-),以水-氢氧化钠为90%︰10%为淋洗液,采用Ionpac AS18(2 mm×250 mm)阴离子分析柱和电导检测器检测,流速为0.25 mL/min,进样量为10μL,依据保留时间定性,标准曲线法定量。结果:SO42-浓度在1.0~100μg/mL范围内呈良好的线性关系(r=0.9999),测得山药、百合、五加皮的平均回收率分别为94.14%、94.72%和94.15%,检出限为0.02 mg/kg,定量限为0.12 mg/kg。结论:该测定方法简便、快速、灵敏、精密度及重现性良好,准确度较高。
Objective:To establish an ion chromatography(IC)method for the determination of sulfur dioxide(SO2)residues in 26 kinds of Traditional Chinese Medicinal materials.Methods:The samples were extracted by distilliation with acid and absorbed with 3%hydrogen peroxide solution.Test solution was separated by Ionpac AS18 column using NaOH and H2O as eluent and detected with conductivity detector.Velocity of flow was 0.25 mL/min.The injection volume was 10μL.The qualitative analysis was made by comparing the reservation time,quantitative analysis was made by standard curve.Results:The linear range of sulfate(SO42-)radical was between 1.0 and 100μg/mL(r=0.9999),the average recovery of Dioscoreae Rhizoma,Lilii Bulbus and Acanthopanacis Cortex were 94.14%,94.72%and 94.15%.the limit of detection was 0.02 mg/kg,and the limit of quantitation was 0.12 mg/kg.Conclusion:The method is simple,rapid and sensitive.It shows better reproducibility and accuracy.
作者
陶松
刘静
刘玲
TAO Song;LIU Jing;LIU Ling(Department of Pharmacy,The No.1 Hospital Affiliated to Nanchang University,Nanchang 330006,China; Department of Pharmacy,The Tumor Hospital of Jiangxi,Nanchang 330029,China;Department of Pharmacy,The People’s Hospital of Jiangxi,Nanchang 330006,China)
出处
《抗感染药学》
2020年第5期627-631,共5页
Anti-infection Pharmacy
基金
江西省卫生计生委中医药科研基金项目(编号:2016A125)。
关键词
离子色谱法
二氧化硫
检测方法
中药材
Ion Chromatography(IC)
sulfur dioxide
determination
Traditional Chinese medicinal materials
