摘要
目的评估慢性阻塞性肺疾病(COPD)患者炎症和氧化应激状态下呼出气冷凝液(EBC)中超细颗粒物(UFP)水平变化。方法选取2017年6月至2018年12月空军军医大学西京医院收治的符合COPD诊断标准的58例患者为研究对象,另选取同期在该院进行健康体检且无系统性疾病的健康志愿者40例作为对照组。通过常规方法收集EBC,NanoSight纳米颗粒分析仪分析微粒。使用ELISA法测量EBC中羰基和8-羟基脱氧鸟苷(8-OHdG)水平,测定肺活量、肺弥散量。结果COPD组患者血清嗜酸性粒细胞计数(EOS abs)、C反应蛋白(CRP)及乳酸脱氢酶(LDH)水平均高于对照组(P<0.05)。与对照组相比,COPD组患者EBC中UFP水平降低(P<0.05),血清中UFP水平升高(P<0.05),EBC中羰基和8-OHdG水平更高(P<0.05)。EBC中UFP水平与羰基水平呈负相关(P<0.05),与FEV1和肺一氧化碳扩散能力(DLCO)呈正相关(P<0.05)。EBC中低UFP水平(≤0.18×108/mL)和CRP≥5 mg/L是频繁加重表型的独立预测因子。结论EBC中UFP水平反映了气道的炎症状态,上皮细胞通透性的增加可能是COPD患者EBC中UFP水平低和血清中UFP水平高的机制。
Objective To evaluate the changes of ultrafine particles(UFP)in exhaled breath condensate(EBC)under inflammatory and oxidative stress in patients with chronic obstructive pulmonary disease(COPD).Methods A total of 58 patients who met the COPD diagnostic criteria from June 2017 to December 2018 were enrolled in the COPD group.Meanwhile,40 healthy volunteers with a physical examination without systemic disease were selected as the control group.The EBC was collected by the conventional method,and the particles were analyzed using a NanoSight nanoparticle analyzer.EBC carbonyl and 8-hydroxydeoxyguanosine(8-OHdG)levels were measured by ELISA,lung capacity and lung diffusion were measured.Results The serum eosinophil count(EOS abs),levels of C reactive protein(CRP)and lactate dehydrogenase in the COPD group were higher than those in the control group(P<0.05).Compared with the control group,UFP level in EBC was lower in the COPD group(P<0.05),but it was higher in serum(P<0.05);the levels of EBC carbonyl and 8-OHdG were higher(P<0.05).Additionally,UFP level in EBC was negatively correlated with carbonyl(P<0.05)while positively related to FEV1 and lung carbon monoxide diffusion capacity(DLCO,P<0.05).Low UFP(≤0.18×108/mL)and CRP level≥5 mg/L in EBC are independent predictors of frequent exacerbation phenotypes.Conclusion The UFP content in EBC reflects the inflammatory state of the airway.Increased permeability of epithelial cells may be the mechanism of low UFP in EBC and high UFP in serum of COPD patients.
作者
张瑶
陈丽展
吴朔
张艳
欧阳海峰
ZHANG Yao;CHEN Lizhan;WU Shuo;ZHANG Yan;OUYANG Haifeng(Department of Respiratory Medicine,Cardiothoracic Hospital of Xi ′an International Medical Center Hospital,Xi ′an,Shaanxi 710100,China;Xijing Hospital of Air Force Medical University,Xi ′an,Shaanxi 710032,China)
出处
《重庆医学》
CAS
2020年第11期1817-1820,共4页
Chongqing medicine
关键词
呼出气冷凝液
氧化应激
肺疾病
慢性阻塞性
exhaled breath condensate
oxidative stress
pulmonary disease
chronic obstructive
