摘要
目的探讨25-羟维生素D[25-hydroxyvitamin D,25(OH)D]和肿瘤坏死因子-α(tumor necrosis foctor-α,TNF-α)对克罗恩病(Crohn disease,CD)的诊断及辅助判断临床特征的价值。方法选取59例CD患者为观察组(CD组)、34例溃疡性结肠炎患者为鉴别组(UC组)及70名健康体检者(正常对照组),分别检测25(OH)D和TNF-α,采用受试者工作特征(receiver operating characteristic,ROC)曲线评价单项及联合分析对CD的诊断价值。按疾病严重程度、临床特征分别进行分组比较,评价25(OH)D和TNF-α对CD患者病情判断的应用价值。结果CD患者组25(OH)D水平低于UC组和正常对照组,TNF-α水平高于UC组和正常对照组,差异均有统计学意义(P<0.05);25(OH)D和TNF-α联合检测诊断CD的ROC曲线下面积(AUC)为0.901,明显高于单项检测(AUC分别为:0.759和0.848);联合检测的灵敏度和特异性分别为84.7%和88.6%。25(OH)D、TNF-α及联合检测鉴别诊断CD和UC的AUC分别为0.630、0.694、0.730。25(OH)D和TNF-α水平在不同严重程度间的差异有统计学意义(P<0.05),在不同临床特征间的差异无统计学意义(P>0.05)。结论25(OH)D和TNF-α联合检测对CD有较高的诊断价值;可用于辅助诊断CD和判断CD的严重程度。
Objective To evaluate the diagnostic value of 25-hydroxyvitamin D(25(OH)D)and tumor necrosis factor-α(TNF-α)in Crohn′s disease(CD).Methods Fifty-nine patients with CD(the CD group),34 patients with ulcerative colitis(the UC group)and 70 healthy subjects(the normal control group)were selected to detect 25(OH)D and TNF-α,respectively,and the ROC curve was used to evaluate the diagnostic value of single and combined analysis for CD.Patients were divided into groups and compared according to disease severity and clinical characteristics,and the application value of 25(OH)D and TNF-αin clinical characteristics judgment of CD patients was evaluated.Results The level of 25(OH)D in the CD group was lower than that in the UC group and the normal control group,while the level of TNF-αwas higher than that in the UC group and the normal control group(P<0.05).The area under ROC curve(AUC)of 25(OH)D combined with TNF-αwas 0.901,significantly higher than that of single-marker detection(AUC:0.759,0.848,respectively),with the sensitivity and specificity 84.7%and 88.6%,respectively.AUC of 25(OH)D,TNF-αand combined detection for differential diagnosis of CD and UC were 0.630,0.694 and 0.730,respectively.25(OH)D and TNF-αlevels were significantly different in different severity(P<0.05),while no significant difference between different clinical features(P>0.05).Conclusion The combined detection of 25(OH)D and TNF-αhas a high diagnostic value for CD,and can be used to assist the diagnosis of CD and judge the severity of CD.
作者
苏鹏
付杰
刘萍
刘飞
张立群
SU Peng;FU Jie;LIU Ping;LIU Fei;ZHANG Liqun(Department of Clinical Laboratory, Xinqiao Hospital, Army Medical University, Chongqing 400037,China)
出处
《标记免疫分析与临床》
CAS
2020年第5期799-804,共6页
Labeled Immunoassays and Clinical Medicine
基金
国家自然科学基金(编号:81772284)
陆军军医大学青年培育项目(编号:2018XQN12)。
