摘要
目的建立人尿中碘含量的酶标仪自动比色测定方法。方法采用酶标仪自动比色测定法测定人尿中碘含量,对该方法的线性范围、检测限、精密度进行验证;与国家卫生行业标准砷铈催化分光光度法的尿碘测定结果进行比较分析。结果酶标仪自动比色测定法碘标准曲线的线性范围为0~300μg/L,线性相关系数(r)范围为-0.9995^-0.9992,检出限为6.5μg/L;低、中、高3种碘浓度尿样的相对标准偏差(RSD)均<3%,回收率范围为92.2%~109.2%,总平均回收率为99.6%。酶标仪自动比色测定法与砷铈催化分光光度法对40份尿样的尿碘含量检测结果比较,差异无统计学意义(t=1.347,P>0.05);酶标仪自动比色测定法的检测速率是砷铈催化分光光度法的7.5倍。结论酶标仪自动比色测定法应用于尿碘含量测定具有合理的检测线性范围,精密度好,准确度高;且自动化快速比色提高了分析速度,适用于大批量样本检测。
Objective To establish an enzyme-labeled instrument automatic colorimetric method for determination of iodine content in human urine.Methods The enzyme-labeled instrument automatic colorimetric method was used to determine the iodine content in human urine.The linear range,detection limit and precision of the method were verified.And the urine iodine test results were compared with the results tested by national health industry standard as arsenic-cerium catalytic spectrophotometry.Results The linear range of iodine standard curve of the enzyme-labeled instrument automatic colorimetric method was 0-300μg/L,the linear correlation coefficient(r)was-0.9995 to-0.9992,and the detection limit was 6.5μg/L.The relative standard deviation(RSD)of urine samples with low,medium and high iodine concentration were all<3%,the recovery rate ranged from 92.2%to 109.2%,and the total average recovery rate was 99.6%.There was no significant difference in the detection results of iodine content in 40 urine samples between the enzyme-labeled instrument automatic colorimetric method and arsenic-cerium catalytic spectrophotometry(t=1.347,P>0.05);and the detection speed of the enzyme-labeled instrument automatic colorimetric method was 7.5 times of the arsenic-cerium catalytic spectrophotometry.Conclusion The enzyme-labeled instrument automatic colorimetric method has a reasonable linear range,good precision and high accuracy in determination of urinary iodine content,and the enzyme-labeled instrument automatied fast colorimetry has improved the analysis speed,it is suitable for detection of large quantities of samples.
作者
徐署东
许娴
赵立胜
刘婷婷
李卫东
Xu Shudong;Xu Xian;Zhao Lisheng;Liu Tingting;Li Weidong(Department for Endemic and Parasitic Diseases Control and Prevention,Anhui Center for Disease Control and Prevention,Hefei 230601,China)
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2020年第3期221-224,共4页
Chinese Journal of Endemiology
关键词
尿
碘
酶标仪
测定范围
检测限
Urine
Iodine
Enzyme-labeling instrument
Limit of detection
