摘要
硫化氢(H2S)和pH在环境和细胞内的许多过程中起着至关重要的作用.为了更好地研究其功能,需要一种快速、灵敏地检测H2S和pH的荧光探针.设计合成了以萘二酰亚胺为荧光基团,叠氮为识别位点,基于分子内电荷转移(ICT)机制的反应型荧光探针L.该探针L在较宽的pH范围(4~11)内稳定性好,对H2S的选择性高,响应速度快(3 min),检测限(1.18μmol L^-1)较低,在0~20μmol L^-1的范围内,荧光强度与H2S浓度呈良好的线性关系(R^2=0.99823).此外,当体系中存在30倍的H2S时,在2≤pH≤6.5的范围内,L对pH变化显示线性关系(R^2=0.98764),可用作pH探针.该探针在检测H2S和30倍H2S存在条件下研究细胞微环境pH变化的方法,在分析检测和病理分析等方面具有潜在的应用前景.
Hydrogen sulfide(H2S)and pH play a most important role in vivo and environment.A fluorescent probe for fast and sensitive detection of H2S and pH are of vital importance for research their functions.A naphthalimide-based fluorescent probe L has been designed and synthesized based on intramolecular charge transfer(ICT)mechanism by utilizing azide as recognition site.The probe L has demonstrated good stability in the pH range of 4~11.It showed satisfactory sensitivity and rapidly response(less than 3 min)to H2S.An excellent linear relationship(R^2=0.99823)displayed in the range of H2S concentrations from 0μmol L^-1 to 20μmol L^-1 and the detection limit was 1.18μmol L^-1.Furthermore,in the present 30 equiv.H2S,it was successfully applied to detection of pH with good linear relationship(R^2=0.98764)in the pH range of 2~6.5.This methods provide a reference for detection of H2S and pH value in the presence of 30 equiv.H2S,which have potential application prospects in analytical detection and pathological analysis.
作者
朱继华
张浩
刘敏
刘旌江
廖原
权正军
王喜存
Zhu Jihua;Zhang Hao;Liu Min;Liu Jingjiang;Liao Yuan;Quan Zhengjun;Wang Xicun(College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070.;College of Chemistry and Chemical Engineering,Longdong University,Qingyang,Gansu 745000)
出处
《有机化学》
SCIE
CAS
CSCD
北大核心
2020年第4期1043-1049,共7页
Chinese Journal of Organic Chemistry
基金
陇原青年创新创业人才工程(No.2019-39)
甘肃省高等学校科研(No.2018A-004)资助项目.
关键词
硫化氢
PH
荧光探针
理论计算
ICT机制
hydrogen sulfide
pH
fluorescent probe
theoretical calculations
ICT mechanism
