摘要
为研究松花粉的抗血小板及抗血栓作用,采用大鼠急性血瘀模型研究松花粉对血流变学的影响和对血小板聚集的影响,采用大鼠动-静脉血栓模型研究松花粉对血栓形成、COX-1和COX-2的表达以及对TXB 2的含量和TXB 2/6-keto-PGF 1α比值的影响.结果显示:松花粉可以抑制ADP诱导的血小板聚集,降低全血粘度、血浆粘度和红细胞压积水平;同时,松花粉可以显著降低血栓重量以及选择性抑制血管壁COX-2的表达,而不影响COX-1的表达,并且能够降低TXB 2的含量以及使TXB 2/6-keto-PGF 1α的比值趋于平衡.松花粉具有较好的抗血小板和抗血栓的作用,并且该作用与其能够高度选择性抑制COX-2的表达和调节TXB 2/6-keto-PGF 1α的平衡有关.
To investigate the antiplatelet and antithrombotic effects of pine pollen,this study tests the effects of pine pollen on hemorheology and platelet aggregation by using an acute blood stasis model in rats.Furthermore,after giving pine pollen treatment,thrombus formation,COX-1,COX-2,TXB 2,and TXB 2/6-keto-PGF 1αare measured in the rat model of arteriovenous shunt.Experimental results show that pine pollen can inhibit ADP-induced platelet aggregation and also significantly reduce whole blood viscosity,plasma viscosity,and hematocrit values.Pine pollen can significantly reduce the weight of the thrombus and selectively inhibit the expression of COX-2 in the vessel wall without affecting the expression of COX-1,it can also reduce the content of TXB 2 and regulate the balance of TXB 2/6-keto-PGF 1α.Thus,pine pollen has good antiplatelet and antithrombotic effects,which may be related to its ability to highly selectively inhibit the expression of COX-2 and regulate the balance of TXB 2/6-keto-PGF 1α.
作者
李新
冀凯
杜世豪
滕莉
王增阳
刘彦君
齐栋
范华英
LI Xin;JI Kai;DU Shi-hao;TENG Li;WANG Zeng-yang;LIU Yan-jun;QI Dong;FAN Hua-ying(School of Pharmacy,Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Key Laboratory of Molecular Pharmacology and Drug Evaluation(Yantai University),Ministry of Education,Yantai University,Yantai 264005,China;Department of Pharmacy,Yantaishan Hospital,Yantai 264000,China;Department of Nephrology,Yuhuangding Hospital/Qingdao University,Yantai 264000,China)
出处
《烟台大学学报（自然科学与工程版）》
CAS
2020年第2期182-187,共6页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
山东省自然科学基金资助项目(ZR2018MH039).
关键词
松花粉
抗血小板
抗血栓
血液流变学
pine pollen
antiplatelet
antithrombotic
hemorheology
