摘要
目的探讨黄芪、莪术配伍联合顺铂(DDP)影响肿瘤新生血管生成的作用机制。方法构建人肝癌裸鼠原位移植瘤模型。成瘤后,将其随机分为8组:模型对照组、阳性对照组(DDP)、黄芪莪术配伍高剂量组(H)、黄芪莪术配伍中剂量组(M)、黄芪莪术配伍低剂量组(L)、黄芪莪术配伍高剂量+顺铂组(H+DDP)、黄芪莪术配伍中剂量+顺铂组(M+DDP)、黄芪莪术配伍低剂量+顺铂组(L+DDP),每组8只,给药15 d,Western Blot及Real-time RT-PCR检测肿瘤组织中TF、HGF、FVⅡ蛋白及mRNA的表达。结果与模型对照组相比,H、M、H+DDP、M+DDP组TF、HGF、FVⅡ蛋白及mRNA表达均显著下降(P<0.01),并与剂量有一定的正相关性。与DDP组相比,H+DDP组TF、HGF、FVⅡ蛋白及mRNA表达降低(P<0.01)。结论黄芪、莪术配伍可能是通过下调TF、HGF、FVⅡ蛋白及mRNA表达对肿瘤新生血管生成产生影响。
Objective To study themechanisms of Huangqi(radix astragali) and Ezhu(curcuma) and cisplatin(DDP) on tumorangiogenesis. Method Human hepatocarcinoma cells HepG2 were transplanted into nudemice to form the study model, and the modelmice were divided at random into model group, DDP group, high dosage of Huangqi(radix astragali) and Ezhu(curcuma) group(H group), intermediate dosage of Huangqi(radix astragali) and Ezhu(curcuma) group(M group), low dosage of Huangqi(radix astragali) and Ezhu(curcuma) group(L group), high dosage of Huangqi(radix astragali) and Ezhu(curcuma) and cisplatin group(H+DDP group), intermediate dosage of Huangqi(radix astragali) and Ezhu(curcuma) and cisplatin group(M+DDP group), low dosage of Huangqi(radix astragali) and Ezhu(curcuma) and cisplatin group(L+DDP group),8 ineach group. The expressions of TF, HGF, FVⅡ protein and mRNA in tumor tissues were detected by Western Blot and Real-time RT-PCR. Result Compared with the model group, the expressions of TF, HGF, FVⅡ protein and mRNA decreased significantly in H, M,H+DDP, M+DDP groups(P<0.01).It was positively correlated with the dose.Compared with DDP group, the expressions of TF, HGF, FVⅡ protein and mRNA obviously decreased in H+DDP group(P<0.01).Conclusion Compatibility of Huangqi(radix astragali) and Ezhu(curcuma)may affecttumorangiogenesis by down-regulating the expressions of TF, HGF, FVⅡ protein and mRNA.
作者
臧文华
胡久略
唐德才
尹刚
郝鹏飞
丁生晨
ZANG Wenhua;HU Jiulue;TANG Decai;YIN Gang;HAO Pengfei;DING Shengchen(ZHANG Zhongjing College of Traditional Chinese Medicine,Nanyang Institute of Technology,Nanyang 453000,Henan,China;Henan Key Laboratory of ZHANG Zhongjing Formulae and Herbs for Immuno regulation,Nanyang 453000,Henan,China;Nanjing University of Chinese Medicine,Nanjing 210046,Jiangsu,China)
出处
《中华中医药学刊》
CAS
北大核心
2020年第3期100-103,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金(81503270,81503267)
国家自然科学基金面上项目(81073072,81373990)
河南省科技厅科技攻关项目(182102310637)
河南省高等学校重点科研项目(15A360011)
河南省中医药科学研究专项(2015ZY02004)。
关键词
黄芪
莪术
肝细胞癌
TF
HGF
FVⅡ
Huangqi(radix astragali)
Ezhu(curcuma)
hepatocellular carcinoma
TF
HGF
FVⅡ
