摘要
选取山东蓝思种业股份有限公司选育并初步认定的"非洲猪瘟抗性猪"蓝思白2号(LS-2)猪,通过实验室非洲猪瘟病毒(ASFV)强毒攻击试验,分析其抗感染应答特征。结果表明,LS-2猪对口服感染106.0TCID50ASFV SY18株具有明显抗性:6头LS-2猪中5头健活,仅在血液中出现间歇、短暂和低水平的病毒血症,并呈现较低程度的发热,随后恢复正常,从12 d开始出现针对病毒结构蛋白p54抗体,之后抗体水平持续升高;1头延迟发病死亡。而普通家猪于感染后3 d开始出现持续发热症状和病毒血症,10 d内全部死亡,期间p54抗体水平一直处于阴性或极低水平。抗性猪和普通家猪炎性因子的检测结果显示,主要抗炎因子如TGF-β2在LS-2抗性猪未感染前处于较低水平,感染后表现轻微(约1.5~2.0倍)升高,而在普通家猪未感染前处于高水平,感染后则表现为显著下降;在感染后6 d(潜伏后期),抗性猪主要促炎因子TNF-α出现轻微(约1.5~2.0倍)升高,而普通家猪则呈现数十至数百倍的显著增高。至试验结束时,LS-2猪临床表现正常,剖检未见内脏器官外观异常,实时PCR于肺、心、骨髓、胸腺等可检出ASFV核酸,但病毒分离结果呈阴性。组织切片染色显示,LS-2猪脾脏和肾脏结构正常,仅肺脏中肺泡呈现轻微融合、扩张。以上结果表明,和普通家猪相比,LS-2猪对ASFV人工感染具有明显抗性,该类型猪具有重大推广意义。
Lansibai-2(LS-2)pigs,which are bred by Shandong Landsee Genetics Co.,Ltd.,and initially thought to be African swine fever resistant,have been confirmed to possess the characteristics of anti-African swine fever virus(ASFV)infection through a trial in P3 laboratory.The results showed that LS-2 had obvious resistance to the oral challenge of virulent ASFV SY18 strain with dosage of 106.0 TCID50,out of 6 LS-2,5 pigs survived,with intermittent,transient and low level viremia,mild fever,and recovered.Antibodies against the viral structural protein p54 began to appear on 12 dpi and continued to rise until the observation end;1 out of 6 died after a delayed disease onset.While the common domestic pigs began to develop fever and viremia on the 3 rd day post infection,and all died within 10 d,during which the p54 antibody was negative or very low.The main anti-inflammatory factors,such as TGF-β2,were at a low level before infection in LS-2 pigs,and showed a slight increase of 1.5-2.0 times post infection,however,in common domestic pigs,it was at a high level before infection and decreased significantly post infection.While the main proinflammatory factor TNF-αin LS-2 pigs increased slightly(1.5-2.0 times)on 6 dpi,the common domestic pig showed significant increase of tens to hundreds of times than before infection.By the end of the trial,LS-2 pigs showed normal clinical performance,but ASFV DNA could be detected by real-time PCR,nonetheless virus isolation was negative.Tissue pathological sections showed that the spleen,heart and kidney of LS-2 were structurally normal,and the lungs become slightly thicken in alveolar diaphragm with a small amount of inflammatory cell infiltration.The above results showed that LS-2 had obvious resistance to ASFV infection compared with the common domestic pigs.This kind of pig is of significant importance for commercialization.
作者
陈腾
陈其美
葛蕾
刘泽云
周鑫韬
齐宇
缪发明
吴添文
王龙
杨金金
高玉伟
杨金梅
岳慧贤
王立冬
韩桪
温兴钰
王洋
米立娟
蒋依倩
张守峰
李奎
蔡雪辉
葛长利
扈荣良
CHEN Teng;CHEN Qi-mei;GE Lei;LIU Ze-yun;ZHOU Xin-tao;QI Yu;MIAO Fa-ming;WU Tian-wen;WANG Long;YANG Jin-jin;GAO Yu-wei;YANG Jin-mei;YUE Hui-xian;WANG Li-dong;HAN Xun;WEN Xing-yu;WANG Yang;MI Li-juan;JIANG Yi-qian;ZHANG Shou-feng;LI Kui;CAI Xue-hui;GE Chang-li;HU Rong-liang(Military'Veterinary Institute Academy of Military Medical Science,PLA Academy of Military Science,Changchun 130122.China;Shandong Lansi Seed Co.,Ltd.,Rizhao,Shandong 052109,China;Beijing Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Scienices,Beijing 100000,China;Harbin Institute of Veterinary Medicine,Chinese Academy of Agricultural Sciences,Harbin 150030,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2020年第4期665-672,共8页
Chinese Journal of Veterinary Science
基金
国家重点研发计划“烈性外来动物疫病防控与高效安全养殖综合技术研发”资助项目(2017YFD0502302)
“非洲猪瘟等外来动物疫病防控科技支撑计划”资助项目(2018YFC0840400)。
关键词
蓝思白2号猪
非洲猪瘟
抗性
鉴定
p54抗体
Lansibai-2 pigs
African swine fever
resistance
identification
p54 antibody
