摘要
目的探讨钩端螺旋体(简称钩体)疫苗免疫豚鼠后所诱导的抗体水平及动态变化。方法筛选检测抗原,利用ELISA法分析来源于不同厂家的不同批次的钩体疫苗免疫豚鼠后所诱导抗体反应,进一步利用钩体菌攻击豚鼠动物模型,探讨研究钩体疫苗诱导的抗体反应与其保护性的相关性。结果来源于钩体LigA蛋白和腐生型PatocⅠ抗原与钩体疫苗免疫豚鼠血清无明显抗原抗体反应。选用钩体疫苗所含的相应血清群菌种的菌体蛋白作为检测抗原,均呈现明显IgG和IgM抗体反应,其中抗原特异性IgG抗体在末次免疫后35 d达到峰值,而抗原特异性IgM抗体的最高峰为末次免疫后11 d。动物攻击试验结果表明,与3倍及9倍疫苗稀释免疫组比较,原倍钩体疫苗免疫动物血清呈现显著的IgG和IgM抗体反应,具有良好的保护性。结论获得了钩体疫苗诱导抗体动态变化规律,初步证实了钩体疫苗诱导血清抗体反应与其保护性具有一定相关性,这些研究为后续钩体疫苗效力替代方法研究奠定基础。
Objective To investigate the dynamic changes of antibodies induced by leptospiral vaccines.Methods Antigens for antibody detection were screened out.ELISA was used to analyze antibody responses induced at different time points after immunizing guinea pigs with different batches of leptospiral vaccines from different manufacturers.To investigate the relationship between antibody responses induced by leptospiral vaccines and their protective effects in animal model,guinea pigs were challenged with Leptospira after immunization.Results There was no significant antigen-antibody reaction between the LigA protein or PatocⅠantigen and the serum samples of guinea pigs immunized with leptospiral vaccines.Notable IgG and IgM antibody reactions were observed in all vaccination groups when using bacterial proteins from seven Leptospira reference strains which were used for the preparation of leptospiral vaccines as envelope antigens.Antigen-specific IgG antibodies peaked at 35 d after the last immunization,and the highest peak of antigen-specific IgM antibodies was reached 11 d after the last immunization.Results of the challenge test showed that non-diluted leptospiral vaccines induced significant IgG and IgM antibody reactions in guinea pigs as compared with those diluted three or nine times,showing good protective effects.Conclusions Analysis of the dynamic changes of antibodies induced by leptospiral vaccines revealed that there was correlation between the induced serum antibody responses and the protective effects.This study provided reference for further study on alternative methods for evaluating leptospiral vaccine potency.
作者
张影
徐颖华
刘向芹
张金龙
陈质斌
王国柱
辛晓芳
曾明
Zhang Ying;Xu Yinghua;Liu Xiangqin;Zhang Jinlong;Chen Zhibin;Wang Guozhu;Xin Xiaofang;Zeng Ming(Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products,National Institutes for Food and Drug Control,Beijing 102269,China;National Center for Medical Culture Collections,Beijing 102629,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2019年第11期864-868,共5页
Chinese Journal of Microbiology and Immunology
基金
国家863计划(2012AA02A402)
国家自然科学基金(81401249)。
