摘要
应用HPLC方法测定党参炔苷单体及党参药材提取液经不同条件(温度、受热时间、光照、pH等)处理后党参炔苷的含量变化,考察各种因素对其主要活性成分化学稳定性的影响。结果党参炔苷单体在60℃以下稳定性良好,在60℃以上其含量随温度的升高及受热时间的延长而显著降低(P<0.05);对光照敏感,80 Klx光照72 h后含量明显降低(P<0.05);在实验设计的不同酸碱度溶液中的稳定性良好,但pH>12时,含量逐渐降低。结果表明,党参炔苷对温度及光照敏感,且在党参药材提取液中的稳定性较单一成分(单体)更好。在药材加工处理、饮片炮制及中成药生产等过程中应注意控制温度、光照及处理时间。
HPLC method was used to monitor the content changes of lobetyolin after being treated with different conditions(temperature,heating time,light,pH,etc.).Result showed the stability of lobetyolin was good under 60 ℃.When the temperature was over 60 ℃,the content of lobetyolin decreased significantly with the increase of temperature and the extension of heating time(P<0.05).After 72 hours of irradiation with 80 Klx,the content of lobetyolin decreased significantly(P<0.05),which would also decrease in the solution with pH>12.In conclusion,the stability of the pure product of lobetyolin was not as good as that in the extract of Codonopsis pilosula,temperature and light had significant effects on the stability of lobetyolin.
作者
廖江敏
李林玉
瞿云安
杨雪峰
杨军宣
LIAO Jiang-min;LI Lin-yu;QU Yun-an;YANG Xue-feng;YANG Jun-xuan(TCM School of Chongqing Medical University,Chongqing 400016,China;Chengdu Jingfu Pharmaceutical Technology Co.,Ltd.,Chengdu 610041,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第3期385-388,434,共5页
Natural Product Research and Development
基金
国家药典委员会项目(ZG2016-2-06)
重庆市卫生和计划生育委员会中医药科技项目(ZY201702132)。
