摘要
该研究探讨了泛素样含PDH和环指域1(UHRF1)对甲状腺乳头状癌(papillary thyroid carcinoma,PTC)细胞增殖、侵袭和迁移的影响。应用Real-time PCR检测正常甲状腺细胞Nthyori3-1、甲状腺乳头状癌细胞BCPAP和K1中UHRF1 mRNA、miR-206和ERαmRNA的表达水平;Real-time PCR检测UHRF1过表达或干扰对miR-206和ERαmRNA的表达影响;MTT、Transwell检测UHRF1过表达或干扰对Nthy-ori3-1、BCPAP、K1细胞增殖、侵袭和迁移影响;Western blot和双荧光素酶报告实验分析miR-206与ERα的靶向关系。结果表明,与Nthy-ori3-1细胞相比,BCPAP和K1细胞中UHRF1 mRNA和ERαmRNA表达水平显著增高(P<0.05),而miR-206表达水平显著降低(P<0.05);UHRF1过表达或干扰处理细胞后,miR-206表达水平与之变化趋势相反,而ERα表达水平与之变化趋势相同;UHRF1促进Nthy-ori3-1、BCPAP和K1细胞增殖、侵袭和迁移;Western blot和双荧光素酶报告实验证实,miR-206靶向ERα基因并抑制其表达。以上结果说明,UHRF1可通过miR-206调控ERα表达,促进甲状腺乳头状癌细胞增殖、侵袭和迁移。
The aim of this study was to investigate the effects of UHRF1(ubiquitin-like with PDH and ring finger domains 1)on proliferation,invasion and migration of PTC(papillary thyroid carcinoma)cells.The expression of UHRF1 mRNA,miR-206 and ERαmRNA in normal thyroid cells Nthy-ori3-1,thyroid papillary carcinoma cells BCPAP and K1 were detected by Real-time PCR.Real-time PCR was adopted to detect the expression of miR-206 and ERαmRNA after UHRF1 overexpression or interference treatment.After treatment of cells with overexpressed or interfered UHRF1,proliferation was examined by MTT while invasion and migration were determined by Transwell.Western blot and dual-luciferase reporter assay were used to verify the relationship between miR-206 and ERα.The results showed that the expression of UHRF1 mRNA and ERαmRNA in BCPAP and K1 cells was significantly higher than that in Nthy-ori3-1 cells(P<0.05),while the expression of miR-206 was significantly lower than that in Nthy-ori3-1 cells(P<0.05).After the cells were overexpressed or interfered by UHRF1,the expression level of miR-206 showed the opposite trend,while the expression level of ERαshowed the same trend.UHRF1 promoted the proliferation,invasion and migration of Nthy-ori3-1,BCPAP and K1 cells.Western blot and dual-luciferase reporter assay confirmed that miR-206 targets ERαgene and inhibits its expression.Taken together,UHRF1 may promote the proliferation,invasion and migration of thyroid papillary carcinoma cells through the regulation of ERαexpression by miR-206.
作者
许琳婉
刘革力
苟茜
邱伊波
刘智敏
XU Linwan;LIU Geli;GOU Xi;QIU Yibo;LIU Zhimin(Institute of Molecular Medicine and Cancer,Chongqing Medical University,Chongqing 400016,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2020年第2期248-255,共8页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:81272937)资助的课题。
