摘要
傅里叶变换红外光谱(FTIR)成像技术可同时获得组织样本的空间分布信息和红外光谱信息。本文利用FTIR成像技术对离体原位乳腺组织样本进行原位研究,通过特征谱带吸光度比值(面积比值)图像结合FTIR光谱分析研究健康和癌变乳腺组织的差异。发现乳腺癌组织的特征带吸光度比值A1087/A1455、A1238/A1455、A1550/A1455、A1650/A1455相对于健康组织明显增大,A1160/A1087、A1740/A1550则减小。研究结果论证了乳腺组织癌变过程中大分子含量的变化规律,体现了FTIR成像技术在乳腺癌诊断方面的准确性、直观性和可行性,其有潜力成为基础研究和临床诊断的一种准确有效的技术手段。
Fourier transform infrared spectroscopy(FTIR)imaging technique can be used to simultaneously obtain spatial distribution and infrared spectral information of samples.In this study,when in vitro breast tissue samples were investigated by using FTIR imaging,and the differences between healthy and cancerous breast tissues were compared by characteristic band absorbance ratio(area ratio)images combining with FTIR spectroscopy analysis.It was found that,compared with healthy tissues,the characteristic band absorbance ratios in cancerous tissues significantly increased in the case of A1087/A1455、A1238/A1455、A1550/A1455、A1650/A1455,and reduced in the case of A1160/A1087、A1740/A1550.The results demonstrated that the change rule of bio-macromolecule contain in carcinogenesis of breast tissue,which reflected the accuracy,visualization and feasibility of FTIR imaging technique in the diagnosis of breast cancer.Therefore,FTIR imaging has potential to become an accurate and effective technical means for basic research and clinical diagnosis.
作者
陆燕飞
朱勇康
赵远
徐新宇
尹建华
LU Yanfei;ZHU Yongkang;ZHAO Yuan;XU Xinyu;YIN Jianhua(College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China;Department of Pathology, Jiangsu Cancer Hospital, Nanjing, 210009, China)
出处
《光散射学报》
2020年第1期78-83,共6页
The Journal of Light Scattering
基金
国家自然科学基金(61378087)
江苏省六大人才高峰计划(SWYY-034)
江苏省研究生科研与实践创新计划项目(KYCX18_0321)。
关键词
傅里叶变换红外光谱成像
乳腺癌
特征谱
吸光度比
Fourier transform infrared spectroscopy imaging
Breast cancer
characteristic band
absorbance ratio
