摘要
采取在体外培养胃癌细胞株(SGC7901),将培养细胞涂于BaF2窗片的方法,应用美国Nicolet(尼高力)公司Magna750-Ⅱ型红外光谱仪对细胞株进行透射红外光谱检测,得到了时间轴上体外培养细胞株的光谱图,将特征性光谱与胃癌组织光谱进行了比对。发现胃癌细胞株红外光谱特征与相应癌组织光谱特征存在异同,说明细胞胃癌组织所表现的红外光谱特征可以反映胃癌细胞本身的红外光谱特征,同时也具有自身的复杂特点。实验为红外光谱法诊断胃癌奠定了细胞学基础。
The aim of this research is to establish the cell basis for the carcinoma tissue diagnosis by exploring a method to obtain the FTIR （Fourier transform infrared） spectra of the cultured carcinoma cells with FTIR spectroscopy and investigating the special spectral features of the carcinoma cells compared with the carcinoma tissues. In the present paper, the gastric carcinoma tissues confirmed by histology were measured using a Nicolet Magna750-Ⅱ FTIR spectrometer and the corresponding FTIR spectra were obtained. The cultured gastric carcinoma cells （SGC7901） were centrifuged to provide a small pellet of cells for FTIR analysis. The cell pellet was then placed on a specially designed salt plate made of BaF2. Then the infrared spectra were recorded by the same equipment. Based on the previously established criteria, a comparative study was subsequently carried out between the spectra of the cultured carcinoma cells （SGC7901） and that of the corresponding gastric tissues. Several infrared spectral features of the carcinoma cells were obtained： the different bands between cells and tissues locate in the range of 3 000-3 600 cm-1 and 1 640 cm-1 which are the range of the hydroxy stretching and blending bands of H2O. There are more H2O out of carcinoma cells in carcinoma tissues, so the strong bands of H2O cover the distinctive bands of carcinoma cells in carcinoma tissues. Although the carcinoma tissue is more complicated, which might originate from the intrinsic complexity of the tissue, the results suggest that the spectral features of the carcinoma cells can be well reflected by that of the carcinoma tissue. This study shows that the diagnosis of carcinoma tissue by FTIR method exhibits sufficient cell basis.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2008年第1期51-54,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(30500119)资助
