摘要
目的基于内毒素血症探讨皂术茵陈方防治非酒精性脂肪性肝炎(NASH)大鼠的影响及其作用机制。方法采用随机数字表法将40只大鼠分为5组,即正常组、模型组、皂术茵陈方组、盐酸吡格列酮组及培菲康组,每组8只。除正常组外,其余32只大鼠采用高脂饮食16周建立大鼠NASH模型,在造模第9周开始,皂术茵陈方组给予皂术茵陈方水提液60 mg/(kg·d)灌胃,盐酸吡格列酮组给予盐酸吡格列酮10 mg/(kg·d)灌胃,培菲康组给予培菲康210 mg/(kg·d)灌胃,正常组及模型组均给予双蒸水10 mL/(kg·d)灌胃,共治疗8周。第16周末经腹主动脉取血,生化法检测肝组织甘油三酯(TG)水平,苏木精-伊红(HE)染色观察肝组织病理学变化。终点显色法检测血浆内毒素(LPS)含量。ELISA法检测肝组织肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6等炎症因子的表达水平。结果与正常组比较,模型组大鼠肝组织显示出典型的NASH组织学特征,出现重度脂肪变性,不同程度的炎细胞浸润和坏死灶。大鼠肝湿重、肝指数、肝脏TG含量、血浆LPS水平、肝组织TNF-α、IL-1β、IL-6等炎症因子水平均有不同程度的增高(P<0.05,P<0.01)。经皂术茵陈方干预后,肝细胞脂肪变性、炎症浸润较模型组减轻,大鼠肝湿重、肝指数、肝脏TG含量、血浆LPS水平、肝组织TNF-α、IL-1β、IL-6等炎症因子水平较模型组有不同程度的下降(P<0.05,P<0.01)。结论皂术茵陈方能显著降低肝脏TG含量、血浆LPS水平,肝组织TNF-α、IL-1β、IL-6等炎症因子的表达。提示皂术茵陈方治疗NASH的作用机制与改善内毒素血症,减少内源性LPS产生、降低炎症因子的表达有关。
Objective To observe the efficacy and mechanism of Zaozhu Yinchen Recipe(ZZYCR) for preventing and treating non-alcoholic steatohepatitis(NASH) of rats based on the endotoxemia. Methods Totally 40 rats were divided into the normal group, the model group, the ZZYCR group, Pioglitazone Hydrochloride Tablets group, and Bifidobacterium group by random digit table, 8 in each group. NASH rat model was established in 32 rats except those in the normal group using high fat diet for 16 weeks. Starting from the 9 th week of modeling, rats in the ZZYCR group were administered with ZZYCR decoction [60 mg/(kg·d)] by gastrogavage. Those in Pioglitazone Hydrochloride Tablets group were administered with Pioglitazone Hydrochloride Tablets [10 mg/(kg·d)] by gastrogavage. Those in the Bifidobacterium group were administered with Bifidobacterium [210 mg/(kg·d)] by gastrogavage. Double distilled water [10 mL/(kg·d)] was administered to rats in the normal group and the model group by gastrogavage. The therapeutic course for all was 8 weeks. Blood was collected from abdominal aorta at the end of 16 weeks. The level of triglyceride(TG) was detected by biochemical method. The pathological changes of liver and intestinal tissues were observed by HE staining. End point chromogenic assay was used to detect the lipopolysaccharide(LPS) level in plasma. The expression levels of inflammatory factors such as tumor necrosis factor-α(TNF-α), IL-1β, and IL-6 in liver tissues were detected by ELISA. Results Compared with the normal group, typical histological features of NASH occurred in the liver tissue of rats in the model group. They showed severe fat degeneration and different degrees of inflammatory cell infiltration and necrosis foci. The levels of liver wet weight, liver index, liver TG content, plasma LPS, the levels of inflammatory factors such as TNF-α, IL-1β, and IL-6 were significantly elevated to various degrees in the model group(P<0.05, P<0.01). After intervened with ZZYCR, steatosis of liver cells and infiltration of inflam
作者
陈平
许玲夏
周志佳
杨青青
苏航
郭蓁萤
王书宇
陈建虎
梁惠卿
赖鹏华
张绍良
李晓英
陈少东
CHEN Ping;XU Ling-xia;ZHOU Zhi-jia;YANG Qing-qing;XU Hang;GUO Zhen-ying;WANG Shu-yu;CHEN Jianhu;LAING Hui-qing;LAI Peng-hua;ZHANG Shao-liang;LI Xiao-ying;CHEN Shao-dong(Faculty of Traditional Chinese Medicine,School of Medicine,Xiamen University,Fujian,361102;Center for Liver Disease,Xiamen Hospital of Traditional Chinese Medicine,Fujian,361009)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2020年第4期485-489,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81503529,No.81673660,No.81873242)
福建省科技计划引导性项目(No.2016D012)
厦门市科技惠民计划项目(No.3502Z20174028)
厦门市重要重大疾病联合攻关项目(No.3502Z20179047)
福建省卫生计生医学创新科研人才培养项目(No.2018-CXB-28)
全国第六批老中医药专家学术经验继承工作(2017年)。
