摘要
目的:构建小鼠股骨远端骨缺损植入模型.方法:8周龄雌性C57BL/6J小鼠股骨远端钻开1 mm直径的孔洞;将直径为1 mm、长度为5 mm的磺化聚醚醚酮植入小鼠股骨骨髓腔内;观察小鼠术后运动情况;术后2周,取材观察植入材料位置;使用micro-CT扫描三维重建小鼠骨微结构.结果:小鼠术后腿部行动自由,健康状况良好且存活至取材,取材后观察植入材料无松动脱出,股骨干完好未见缺损;micro-CT三维重建结果显示植入物位置正确.结论:使用该方法可成功构建小鼠股骨远端缺损模型.
Objective:Establishing a mouse femur bone defection model.Methods:Three 8-week-old female C57BL/6J mouse were used in this experiment.A hole with 1 mm diameter was drilled on the distal femur.Then,the sulfonated polyetheretherketone(PEEK)with 1 mm diameter and 5 mm length was implanted into the femur marrow cavity.Micro-CT was used to reconstruct the bone microstructure and observe the implant position tow weeks later.Results:The health condition of mice with bone defection is good post-surgery and can survive until sacrifice.The movement of them is unlimited.The photos show that the implantation is fine placed in the femur marrow cavity.There is not any obvious damage.3D reconstructed micro-CT images show that the position of implantation is fine.Conclusion:The mouse femur defection model was successfully established in this work.
作者
孙莹霄
廖赟
欧阳丽萍
宋文刚
SUN Yingxiao;LIAO Yun;OUYANG Liping;SONG Wengang(College of Pharmacy,Beihua University,Jilin City 132013,China;Department of Pharmacy,Tongren Hospital Affiliated to Medical College of Shanghai Jiao Tong University ,Shanghai 200336,China)
出处
《吉林化工学院学报》
CAS
2020年第3期38-42,共5页
Journal of Jilin Institute of Chemical Technology
基金
吉林农业大学博士后基金项目
国家自然科学基金(81403029)
上海市科委面上项目(19ZR1449100).
