摘要
目的探究芒针深刺秩边穴对大鼠脊髓损伤后运动功能的影响及可能作用机制。方法选择健康雄性Wister大鼠81只,随机分为正常组、模型组和芒针组(正常组9只,其余两组各36只),采用改良Allen's造模法制备大鼠脊髓中度损伤模型,模型组不做特殊处理,芒针组采用芒针深刺秩边穴,每日1次,每次30 min。分别于术后1 d、3 d、5 d、7 d行BBB(Basso-Beattie-Bresnahan)运动功能评分;术后1 d、3 d、5 d、7 d取受损段脊髓组织行酶联免疫吸附测定(ELISA)、实时荧光定量PCR(RT-qPCR)和苏木素-伊红染色(HE染色)。结果术后5 d和7 d,芒针组大鼠BBB评分高于模型组,差异有统计学意义(P<0.01);脊髓损伤后,模型组和芒针组大鼠脊髓组织中高迁移率族蛋白B1(HMGB1)、核转录因子kB(NF-kB)、白介素-6(IL-6)含量及HMGB1mRNA、NF-kBmRNA、IL-6mRNA水平显著升高(P<0.05);受损的脊髓组织松散,灰质中有许多空洞形成,伴有炎性细胞浸润。芒针治疗后,芒针组大鼠脊髓组织中HMGB1、NF-kB、IL-6含量及HMGB1mRNA、NF-kBmRNA、IL-6mRNA水平较模型组降低,且在3 d、5 d、7 d差异有统计学意义(P<0.05);受损部位的空洞及炎性细胞逐渐减少。结论脊髓损伤后,炎症因子的大量聚集引起级联性炎症反应,影响大鼠运动功能的恢复。芒针的抗炎机制可能包括抑制HMGB1的表达,降低NF-kB信号通路的传导,下调促炎因子IL-6的分泌。
Objective To investigate the effect of deep insertion of an elongated needle into point Zhibian(BL54)on motor function in rats with spinal cord injury and explore its possible mechanism of action.Method Eighty-one male Wister rats were randomized to normal(9 rats),model(36 rats)and elongated needle(36 rats)groups.A rat model of moderate spinal cord injury was made by the modified Allen's method.The model group was not treated.The elongated needle group received deep insertion of an elongated needle into point Zhibian,30 min once daily.The BBB(Basso-Beattie-Bresnahan)locomotor scores were recorded at one,three,five and seven days after the operation.The injured spinal segment tissue was taken for enzyme linked immunosorbent assay(ELISA),real-time fluorescence quantification PCR(RT-qPCR)and hematoxylin-eosin(HE)staining at one,three,five and seven days after the operation.Result At five and seven days after the operation,the BBB score was higher in the elongated needle group of rats than in the model group with a statistically significant difference(P<0.01).In the model and elongated needle groups of rats after spinal cord injury,high mobility group box-1 protein(HMGB1),nuclear transcription factor-κB(NF-κB)and interleukin 6(IL-6)contents and HMGB1 mRNA,NF-κB mRNA and IL-6 mRNA levels increased(P<0.05);the injured spinal cord tissue was loose and many cavities formed in the gray nucleus with inflammatory cell infiltration.After elongated needle treatment,spinal cord tissue HMGB1,NF-κB and IL-6 contents and HMGB1 mRNA,NF-κB mRNA and IL-6 mRNA levels were lower in the elongated needle groups of rats than in the model group with a statistically significant difference at day three,five and seven(P<0.05);cavities and inflammatory cells gradually decreased in the injured part.Conclusion After spinal cord injury,massive accumulation of inflammatory factors causes a cascade of inflammatory reactions and affects the recovery of rat motor function.The anti-inflammatory mechanism of elongated needle puncture may include
作者
吕建兰
胡劲涛
柴乐
钱剑胜
任伟凡
全仁夫
Lü Jian-lan;HU Jin-tao;CHAI Le;QIAN Jian-sheng;REN Wei-fan;QUAN Ren-fu(Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China;Zhejiang University of Traditional Chinese Medicine Jiangnan Hospital,Xiaoshan 311200,China)
出处
《上海针灸杂志》
2020年第4期493-500,共8页
Shanghai Journal of Acupuncture and Moxibustion
基金
浙江省中医药科研计划项目(2016za168)。
关键词
针刺疗法
长针疗法
芒针
脊髓损伤
穴
秩边
炎症反应
高迁移率组蛋白1
核转录因子KB
白介素-6
大鼠
Acupuncture therapy
Long needle therapy
Elongated needle
Spinal cord injury
Point,Zhibian(BL54)
Inflammatory reaction
High mobility group box-1 protein
Nuclear transcription factor-κB
Interleukin 6
Rats
