摘要
采用铜离子与硫离子在溶液中直接反应制备粒径为50~60 nm的硫化铜纳米颗粒(CuS NPs).在CuS NPs表面修饰了聚多巴胺层,并利用聚多巴胺(PDA)的非共价键自聚在聚多巴胺层表面负载DOX,从而得到一种核壳结构纳米粒子——DOX@CuS/PDA(其核为CuS NPs,壳为担载阿霉素的聚多巴胺).研究表明,该硫化铜纳米颗粒具备光热敏感特性,在功率为1 W/cm^2的808 nm近红外光照射下,光热转化率高达34.2%.在不同pH下释放的研究结果表明,在酸性条件下经过72 h体外释放后,DOX@CuS/PDA累积DOX的释放量是其在碱性环境中的6倍,证明了DOX@CuS/PDA拥有明显的pH刺激响应释放机制.在体外癌细胞实验结果中,DOX@CuS/PDA可以将光热治疗与化学药物治疗相互结合,癌细胞存活率仅有27%~33%,有效地提升了肿瘤细胞灭杀效果.本研究对于光热治疗和化学治疗相结合提供了研究方案,并且对于吸附载药以及pH响应药物释放研究提供了新认识.
In recent years, the incidence of malignant tumors is increasing year by year. Therefore, new antitumor drugs and treatment modalities are urgently needed. While, the biggest challenge to oncology drugs is to treat tumors in a safe and effective manner. Currently, most of the cancer patients in treatment are suffering due to side effects of oncology drugs. In order to reduce negative effects of tumor drugs, formulations of multifunctional nanocarriers have attracted attention because of their significant biological activity without damage to normal cells. These include formulations such as drug-loaded silica nanoparticles, photothermal treatment of gold nanoparticles, photodynamic therapy of copper sulfide nanoparticles and other multifunctional nanocomposites.In this study, a novel kind of high photothermal conversion CuS NPs as a pH-sensitive drug release system is going to be introduced. Firstly, CuS NPs with a uniform particle size was prepared by copper ions and sulfur ions in solution. Secondly,the PDA was modified on the surface of CuS NPs and DOX was adsorbed on the outside of PDA via non-covalent selfassembly. Finally, a drug-loaded core-shell nanoparticle DOX@CuS/PDA was obtained, with the core of CuS NPs and the shell of PDA. Further studies on DOX@CuS/PDA were performed to verify its anti-cancer effect.Surface morphology of DOX@CuS/PDA was observed by transport electronic microscope(TEM). TEM images show that DOX@CuS/PDA is uniform with a size of 70±10 nm and appears as a spherical shape with a CuS NPs core. After comprehensive consideration of absorbance test, wavelength penetration capability and equipment, 808 nm near-infrared light was considered to be the most suitable light source. The 200 μg/mL of CuS NPs solution was heated to 54℃ in 120 s under the irradiation of 808 nm near-infrared light with a power of 1 W/cm^2, and the photothermal conversion rate was as high as 34.2%. The temperature of DOX@CuS/PDA was raised to 41.4℃ in the same condition. In vitro release assay, the cumulative re
作者
秦玉杰
韩宇
靳杭
高颖
单晓茜
Yujie Qin;Yu Han;Hang Jin;Ying Gao;Xiaoqian Shan(School of Materials Science and Engineering,Shanghai Institute of Technology,Shanghai 201418,China)
出处
《科学通报》
EI
CAS
CSCD
北大核心
2020年第2期203-212,共10页
Chinese Science Bulletin
基金
上海市自然科学基金(16ZR1436000)
上海应用技术大学产学研平台(10210Q180024-2016xt024)资助。
关键词
纳米硫化铜
聚多巴胺
近红外光热转化
PH响应
copper sulfide nanoparticles
polydopamine
near-infrared photothermal conversion
pH-stimuli response
